Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Stachyose ameliorates myocardial ischemia–reperfusion injury by inhibiting cardiomyocyte ferroptosis and macrophage pyroptosis
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
Inhibiting endoplasmic reticulum stress alleviates perioperative neurocognitive disorders by reducing neuroinflammation mediated by NLRP3 inflammasome activation
IF: 4.8Journal： CNS Neuroscience & Therapeutics (2024)Reactivity：MouseSample Type：hippocampal tissue -
Collagen peptide and taurine synergistically improve hypertensive kidney injury induced by high salt diet through gut-kidney axis
IF: 4.8Journal： Food Bioscience (2024)Reactivity：MouseSample Type：kidney tissue,jejunum tissue -
Codonopsis pilosula polysaccharide alleviates ulcerative colitis by modulating gut microbiota and SCFA/GPR/NLRP3 pathway
IF: 4.8Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：colon tissue -
ATP citrate lyase ablation hampers exocrine regeneration via TLR4/NF-kappaB signaling after acute pancreatitis in mice
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：Plasma -
Protective Effects of Isostrictiniin Against High-Fat, High-Sugar Diet-Induced Steatosis in MASLD Mice via Regulation of the AMPK/SREBP-1c/ACC Pathway
IF: 4.8 -
Relationship Between Metabolomics of T2DM Patients and the Anti-Diabetic Effects of Phellodendri Chinensis Cortex-Anemarrhenae Rhizoma Herb Pair in Mice
IF: 4.8Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
Network pharmacology-based strategy to reveal Acacetin against lipopolysaccharide-induced lung injury
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：lung tissue -
Novel insights into cuproptosis in alcoholic liver disease using bioinformatics analysis and experimental validation
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：Serum -
Taraxasterol extracted from Ixeridium gramineum (Fisch.) Tzvel. attenuated D-GalN/LPS-Induced Fulminant Hepatitis by modulating the JAK/STAT and TNF signalling pathways
IF: 4.8Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：liver tissue
实验操作视频
