Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Formononetin Alleviates Ischemic Acute Kidney Injury by Regulating Macrophage Polarization through KLF6/STAT3 Pathway
IF: 4.8Journal： AMERICAN JOURNAL OF CHINESE MEDICINE (2024)Reactivity：MouseSample Type：serum -
Uncovering the pharmacological mechanisms of Patchouli essential oil for treating ulcerative colitis
IF: 4.8Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：colon tissue -
Biochanin a ameliorates DSS-induced ulcerative colitis by improving colonic barrier function and protects against the development of spontaneous colitis in the Muc2 deficient mice
IF: 4.7Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2024)Reactivity：MouseSample Type：colon tissue -
Salinomycin, a potent inhibitor of XOD and URAT1, ameliorates hyperuricemic nephropathy by activating NRF2, modulating the gut microbiota, and promoting SCFA production
IF: 4.7Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2024)Reactivity：MouseSample Type：serum,kidney tissue -
Comparison of Protective Effects of Polyphenol-Enriched Extracts from Thinned Immature Kiwifruits and Mature Kiwifruits against Alcoholic Liver Disease in Mice
IF: 4.7 -
Regulatory Effect of Fucoidan Hydrolysates on Lipopolysaccharide-Induced Inflammation and Intestinal Barrier Dysfunction in Caco-2 and RAW264.7 Cells Co-Cultures
IF: 4.7 -
Icariin-loaded multilayered films deposited onto micro/nanostructured titanium enhances osteogenesis and reduces inflammation under diabetic conditions
IF: 4.7Journal： Colloid and Interface Science Communications (2024)Reactivity：Mouse -
In-vitro antioxidant and anti-inflammatory potential along with p.o. pharmacokinetic profile of key bioactive phytocompounds of Snow Mountain Garlic: a comparative analysis vis-à-vis normal garlic
IF: 4.6 -
An Amino Acids and Dipeptide Injection Inhibits the TNF-α/HMGB1 Inflammatory Signaling Pathway to Reduce Pyroptosis and M1 Microglial Polarization in POCD Mice: the Gut to the Brain
IF: 4.6Journal： MOLECULAR NEUROBIOLOGY (2024)Reactivity：MouseSample Type：serum -
Omaveloxolone Ameliorates Cognitive Deficits by Inhibiting Apoptosis and Neuroinflammation in APP/PS1 Mice
IF: 4.6Journal： MOLECULAR NEUROBIOLOGY (2024)Reactivity：MouseSample Type：brain tissue
实验操作视频
