Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Betulin alleviates on myocardial inflammation in diabetes mice via regulating Siti1/NLRP3/NF-κB pathway
IF: 3.943Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)PMID：32531709Reactivity：MouseSample Type：serum, heart -
COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice
IF: 3.943Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)PMID：32702600Reactivity：MouseSample Type：Tissue homogenate -
NLRP12 negatively regulates EtOH-induced liver macrophage activation via NF-κB pathway and mediates hepatocyte apoptosis in alcoholic liver injury
IF: 3.943 -
Didymin ameliorates dexamethasone-induced non-alcoholic fatty liver disease by inhibiting TLR4/NF-κB and PI3K/Akt pathways in C57BL/6J mice
IF: 3.943Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)PMID：33182043Reactivity：MouseSample Type：Tissue homogenate -
miR-129-5p alleviates LPS-induced acute kidney injury via targeting HMGB1/TLRs/NF-kappaB pathway
IF: 3.943 -
Myricitrin pretreatment ameliorates mouse liver ischemia reperfusion injury
IF: 3.943 -
Inhibition of HMGB1 involved in the protective of salidroside on liver injury in diabetes mice
IF: 3.943Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)PMID：33217691Reactivity：MouseSample Type：Cell culture supernatant,Serum,Tissue homogenate -
Adiponectin Inhibits NLRP3 Inflammasome Activation in Nonalcoholic Steatohepatitis via AMPK-JNK/ErK1/2-NFκB/ROS Signaling Pathways
IF: 3.900Journal： Frontiers in Medicine (2020)PMID：33251225Reactivity：MouseSample Type：Tissue homogenate(liver tissue) -
Hesperidin alleviates chronic restraint stress and lipopolysaccharide-induced Hippocampus and Frontal cortex damage in mice: Role of TLR4/NF-κB, p38 MAPK/JNK, Nrf2/ARE signaling
IF: 3.881 -
In vitro and in vivo biocompatibility study of surface modified TiN deposited on Ti6Al4V using high-power impulse magnetron sputtering technique
IF: 3.784Journal： SURFACE & COATINGS TECHNOLOGY (2020)Reactivity：MouseSample Type：skin
实验操作视频
