Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Soluble E-cadherin contributes to inflammation in acute lung injury via VEGF/VEGFR2 signaling
IF: 8.2Journal： Cell Communication and Signaling (2025)Reactivity：MouseSample Type：bronchoalveolar lavage fluids (BALF) -
Regulating the balance between GSDMD-mediated pyroptosis and CHMP4B-dependent cell repair attenuates calcium oxalate kidney stone formation
IF: 8.2Journal： International Journal of Biological Sciences (2025)Reactivity：MouseSample Type：serum -
Modulating the Immunosuppressive Tumor Microenvironment and Inhibiting Growth in Mutp53-Driven CRPC via STAT3 Pathway Blockade
IF: 8.2Journal： International Journal of Biological Sciences (2025)Reactivity：MouseSample Type：prostate tumor tissue -
A novel deacetylase inhibitor KLX suppresses liver fibrosis by deacetylating PPARγ through promoting ubiquitination-mediated HDAC1 degradation
IF: 8.0Journal： Science China-Life Sciences (2025)Reactivity：MouseSample Type：serum,liver tissue -
Cornuside alleviates cognitive impairments induced by Aβ1?42 through attenuating NLRP3-mediated neurotoxicity by promoting mitophagy
IF: 7.9 -
Patchouli alcohol from Pogostemon cablin Benth inhibits H1N1 infection by repressing inflammasome and proptosis by targeting ubiquitin specific peptidase USP18
IF: 7.7Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF),serum -
Carboxymethyl Poria cocos polysaccharides protect against septic kidney injury by regulating the Nrf2-NF-κB signaling pathway
IF: 7.7Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)Reactivity：MouseSample Type：serum,kidney tissue -
Astragalus polysaccharide alleviates oxidative stress and senescence in chondrocytes in osteoarthritis via GCN2/ATF4/TXN axis
IF: 7.7Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)Reactivity：Mouse -
Gut commensal Lachnospiraceae bacteria contribute to anti-colitis effects of Lactiplantibacillus plantarum exopolysaccharides
IF: 7.7Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)Reactivity：MouseSample Type：colon tissue -
Akebia trifoliata extracts attenuate liver injury via gut–liver axis in a murine model of nonalcoholic fatty liver disease with low-grade colitis
IF: 7.0Journal： FOOD RESEARCH INTERNATIONAL (2025)Reactivity：MouseSample Type：liver tissue,serum
实验操作视频
