Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Atractylon treatment prevents sleep-disordered breathing-induced cognitive dysfunction by suppression of chronic intermittent hypoxia-induced M1 microglial activation
IF: 2.942Journal： BIOSCIENCE REPORTS (2020)PMID：32490526Reactivity：MouseSample Type：Cell culture supernatant -
Exposure time determines the protective effect of Trichinella spiralis on experimental colitis
IF: 2.914 -
Ablation of GSDMD Improves Outcome of Ischemic Stroke Through Blocking Canonical and Non-canonical Inflammasomes Dependent Pyroptosis in Microglia
IF: 2.889 -
Isomangiferin Attenuates Renal Injury in Diabetic Mice via Inhibiting Inflammation
IF: 2.842Journal： Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2020)PMID：33204133Reactivity：MouseSample Type：Serum,Tissue homogenate -
Curcumin amends Ca2+ dysregulation in microglia by suppressing the activation of P2X7 receptor
IF: 2.795Journal： MOLECULAR AND CELLULAR BIOCHEMISTRY (2020)PMID：31894530Reactivity：RatSample Type：brain, serum -
Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom
IF: 2.762 -
hnRNPK knockdown alleviates NLRP3 inflammasome priming by repressing FLIP expression in Raw264.7 macrophages
IF: 2.753 -
Curcumin prevents osteocyte apoptosis by inhibiting M1-type macrophage polarization in mice model of glucocorticoid-associated osteonecrosis of the femoral head
IF: 2.728Journal： JOURNAL OF ORTHOPAEDIC RESEARCH (2020)PMID：32009245Reactivity：MouseSample Type：peripheral blood -
Sodium P-aminosalicylic Acid Inhibits Manganese-Induced Neuroinflammation in BV2 Microglial Cells via NLRP3-CASP1 Inflammasome Pathway
IF: 2.639Journal： BIOLOGICAL TRACE ELEMENT RESEARCH (2020)PMID：33156491Reactivity：MouseSample Type：Cell sample -
Activation of Paraventricular Melatonin Receptor 2 Mediates Melatonin-Conferred Cardioprotection Against Myocardial Ischemia/Reperfusion Injury
IF: 2.598Journal： JOURNAL OF CARDIOVASCULAR PHARMACOLOGY (2020)PMID：32433359Reactivity：MouseSample Type：PVN tissue
实验操作视频
