Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Subcutaneous administration of casein attenuates atherosclerotic progression in male apoE-/- mice fed with high-fat diet
IF: 1.797 -
Clostridium butyricum protects intestinal barrier function via upregulation of tight junction proteins and activation of the Akt/mTOR signaling pathway in a mouse model of dextran sodium sulfate?induced colitis
IF: 1.785Journal： Experimental and Therapeutic Medicine (2020)PMID：32934675Reactivity：MouseSample Type：Serum -
Impact of Lesinurad and allopurinol on experimental Hyperuricemia in mice: biochemical, molecular and Immunohistochemical study
IF: 1.771 -
Geniposide attenuates dextran sulfate sodium-induced colitis in mice via Nrf-2/HO-1/NF-κB pathway
IF: 1.681Journal： Annals of Palliative Medicine (2020)Reactivity：MouseSample Type：serum,colon tissue -
LncRNA TUG1 reverses LPS-induced cell apoptosis and inflammation of macrophage via targeting MiR-221-3p/SPRED2 axis
IF: 1.516Journal： BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY (2020)PMID：32841583Reactivity：MouseSample Type：Tissue homogenate -
Anti-inflammatory effects of astaxanthin against fungal keratitis
IF: 1.330Journal： International Journal of Ophthalmology (2020)PMID：33214996Reactivity：MouseSample Type：Tissue homogenate(Corneas) -
Gallincin ameliorates colitis-associated inflammation and barrier function in mice based on network pharmacology prediction
IF: 1.287Journal： JOURNAL OF INTERNATIONAL MEDICAL RESEARCH (2020)PMID：33322986Reactivity：MouseSample Type：homogenized colonic tissue -
Human umbilical cord mesenchymal stem cells suppress lung cancer via TLR4/NF-κB signalling pathway
IF: 1.186Journal： BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT (2020)Reactivity：MouseSample Type：Lewis cell-xenograft -
Anti-hypernociceptive effects of methanol extract of Boswellia dalzielii on STZ-induced diabetic neuropathic pain
Journal： Advances in Traditional Medicine (2020)Reactivity：MouseSample Type：serum, sciatic nerves -
Shen-ling-bai-zhu-san ameliorates inflammation and lung injury by increasing the gut microbiota in the murine model of Streptococcus pneumonia-induced pneumonia
Journal： BMC Complementary Medicine and Therapies (2020)PMID：32460745Reactivity：MouseSample Type：Tissue homogenate
实验操作视频
