Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Nicotinamide N-methyltransferase ameliorates renal fibrosis by its metabolite 1-methylnicotinamide inhibiting the TGF-β1/Smad3 pathway
IF: 5.834Journal： FASEB JOURNAL (2022)Reactivity：MouseSample Type：kidney tissue -
High-fat diet aggravates colitis via mesenteric adipose tissue derived exosome metastasis-associated lung adenocarcinoma transcript 1
IF: 5.374Journal： WORLD JOURNAL OF GASTROENTEROLOGY (2022)Reactivity：MouseSample Type：colon tissue -
Sesame oil ameliorates valproic acid-induced hepatotoxicity in mice: integrated in?vivo–in silico study
IF: 5.235Journal： JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (2022)Reactivity：MouseSample Type：serum -
Ameliorative Effects of Peptide Phe-Leu-Ala-Pro on Acute Liver and Kidney Injury Caused by CCl4 via Attenuation of Oxidative Stress and Inflammation
IF: 4.132Journal： ACS Omega (2022)Reactivity：MouseSample Type：liver ,kidney tissue -
Sulfated Galactofucan from Sargassum Thunbergii Attenuates Atherosclerosis by Suppressing Inflammation Via the TLR4/MyD88/NF-κB Signaling Pathway
IF: 3.947 -
Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease
IF: 2.989Journal： Neuropsychiatric Disease and Treatment (2022)PMID：33574670Reactivity：MouseSample Type：Cell lysate -
Down-regulation of KLF9 ameliorates LPS-caused acute lung injury and inflammation in mice via reducing GSDMD expression
IF: 2.957 -
Repression of Toll-like receptor-4 by microRNA-149-3p is associated with smoking-related COPD
IF: 2.893Journal： International Journal of Chronic Obstructive Pulmonary Disease (2022)PMID：28260877Reactivity：MouseSample Type：Cell culture supernatant -
Schistosoma japonicum Eggs Exerts Protective Effects in an Experimental Ulcerative Colitis Model
IF: 2.831Journal： BIOMEDICAL AND ENVIRONMENTAL SCIENCES (2022)Reactivity：MouseSample Type：colon tissue -
Acacetin improves cognitive function of APP/PS1 Alzheimer’s disease model mice via the NLRP3 inflammasome signaling pathway
IF: 1.264Journal： Translational Neuroscience (2022)Reactivity：MouseSample Type：Brain tissue
实验操作视频
