Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Antihypernociceptive, Anxiolytic, and Antidepressant Properties of Aqueous and Ethanol Extracts of Dissotis thollonii Cogn. (Melastomataceae) in Mice
Journal： Advances in Pharmacological and Pharmaceutical Sciences (2020)PMID：33015629Reactivity：MouseSample Type：Serum,Tissue homogenate -
Baicalin ameliorates neuroinflammation-induced depressive-like behavior through inhibition of toll-like receptor 4 expression via the PI3K/AKT/FoxO1 pathway
IF: 5.700Journal： Journal of Neuroinflammation (2019)PMID：31068207Reactivity：MouseSample Type：hippocampus tissue -
Oral administration of 3,3′-diselenodipropionic acid prevents thoracic radiation induced pneumonitis in mice by suppressing NF-kB/IL-17/G-CSF/neutrophil axis
IF: 5.657Journal： FREE RADICAL BIOLOGY AND MEDICINE (2019)PMID：31521664Reactivity：MouseSample Type：Bronchoalveolar Lavage Fluid,Tissue homogenate -
Anti-Neuroinflammatory Effect of Alantolactone through the Suppression of the NF-κB and MAPK Signaling Pathways
IF: 5.656 -
Inhibition of CREB-mediated ZO-1 and activation of NF-κB-induced IL-6 by colonic epithelial MCT4 destroys intestinal barrier function
IF: 5.039 -
Reactive Oxygen Species Are Involved in the Development of Gastric Cancer and Gastric Cancer-Related Depression through ABL1-Mediated Inflammation Signaling Pathway
IF: 4.868Journal： Oxidative Medicine and Cellular Longevity (2019)PMID：31396300Reactivity：MouseSample Type：blood -
Intranasal MMI-0100 Attenuates Aβ1?42- and LPS-Induced Neuroinflammation and Memory Impairments via the MK2 Signaling Pathway
IF: 4.716Journal： Frontiers in Immunology (2019)PMID：31849936Reactivity：MouseSample Type：Tissue homogenate -
Lactobacillus acidophilus Exerts Neuroprotective Effects in Mice with Traumatic Brain Injury
IF: 4.416Journal： JOURNAL OF NUTRITION (2019)PMID：31174208Reactivity：MouseSample Type：Serum,Tissue homogenate -
Modulation of LPA1 receptor-mediated neuronal apoptosis by Saikosaponin-d: A target involved in depression
IF: 4.367 -
Arsenic Trioxide in Synergy with Vitamin D Rescues the Defective VDR-PPAR- Functional Module of Autophagy in Rheumatoid Arthritis
IF: 4.186
实验操作视频
