Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Sinomenine Attenuates Cartilage Degeneration by Regulating miR-223-3p/NLRP3 Inflammasome Signaling
IF: 2.939Journal： INFLAMMATION (2019)PMID：30847744Reactivity：MouseSample Type：Serum,Cell culture supernatant -
Role of Interleukin-17 in Pathogenesis of Intestinal Fibrosis in Mice
IF: 2.937 -
Formononetin Ameliorates Cognitive Disorder via PGC-1α Pathway in Neuroinflammation Conditions in High-Fat Diet-Induced Mice
IF: 2.761Journal： CNS & Neurological Disorders-Drug Targets (2019)PMID：31389320Reactivity：MouseSample Type：hippocampus -
Effect and mechanism of citral against methicillin-resistant Staphylococcus aureus in vivo
IF: 2.422Journal： JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2019)PMID：30891759Reactivity：MouseSample Type：Serum -
Lipofundin Mediates the Major Inhibition of Intravenous Propofol in IL-1β Secretion and Phagocytosis of Staphylococcus aureus-Infected Macrophages
IF: 2.144 -
Antihypernociceptive and neuroprotective effects of Combretin A and Combretin B on streptozotocin-induced diabetic neuropathy in mice
IF: 2.058Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2019)PMID：30759263Reactivity：MouseSample Type：Serum,Spinal cord -
Role of Brd4 in the production of inflammatory cytokines in mouse macrophages treated with titanium particles
IF: 2.041Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2019)PMID：31330113Reactivity：MouseSample Type：Cell culture supernatant -
Hybrids of oxoisoaporphine–tetrahydroisoquinoline: novel multi-target inhibitors of inflammation and amyloid-β aggregation in Alzheimer’s disease
IF: 2.032 -
Kisspeptin?13 inhibits bleomycin?induced pulmonary fibrosis through GPR54 in mice
IF: 1.851 -
Expression and role of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis
IF: 1.189Journal： International Journal of Ophthalmology (2019)PMID：31131225Reactivity：MouseSample Type：Cell culture supernatant
实验操作视频
