Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Antioxidant and anti-inflammatory effects of an ethanol fraction from the Schisandra chinensis baillon hot water extract fermented using Lactobacilius paracasei subsp. tolerans
IF: 0.888Extracellular vesicles from human urine-derived stem cells prevent osteoporosis by transferring CTHRC1 and OPG
Celastrol attenuates ox-LDL-induced mesangial cell proliferation via suppressing NLRP3 inflammasome activation
Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines
IF: 12.354A Redox-Responsive Self-Assembled Nanoprobe for Photoacoustic Inflammation Imaging to Assess Atherosclerotic Plaque Vulnerability
IF: 6.042Journal： ANALYTICAL CHEMISTRY (2018)PMID：30497260Reactivity：MouseSample Type：Cell culture supernatantSpatial Training Ameliorates Long-Term Alzheimer’s Disease-Like Pathological Deficits by Reducing NLRP3 Inflammasomes in PR5 Mice
IF: 5.719Ageratina adenophora induces mice hepatotoxicity via ROS-NLRP3-mediated pyroptosis
IF: 4.122Assessment of novel azaanthraquinone derivatives as potent multi-target inhibitors of inflammation and amyloid-β aggregation in Alzheimer’s disease
IF: 3.929Blocking PI3K/AKT signaling inhibits bone sclerosis in subchondral bone and attenuates post-traumatic osteoarthritis
IF: 3.923SIRT1 activation by butein attenuates sepsis-induced brain injury in mice subjected to cecal ligation and puncture via alleviating inflammatory and oxidative stress
IF: 3.616Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2018)PMID：30336174Reactivity：MouseSample Type：Serum,Tissue homogenate
