Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Upregulated galectin-3 is not a critical disease mediator of cardiomyopathy induced by β2-adrenoceptor overexpression
IF: 3.569Journal： AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY (2018)PMID：29424570Reactivity：MouseSample Type：left ventricle (LV) tissues -
Verapamil Ameliorates Hepatic Metaflammation by Inhibiting Thioredoxin-Interacting Protein/NLRP3 Pathways
IF: 3.519 -
Jinxin oral liquid inhibits human respiratory syncytial virus-induced excessive inflammation associated with blockade of the NLRP3/ASC/Caspase-1 pathway
IF: 3.457Journal： BIOMEDICINE & PHARMACOTHERAPY (2018)PMID：29864921Reactivity：MouseSample Type：Serum,Tissue homogenate -
The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice
IF: 3.457Journal： BIOMEDICINE & PHARMACOTHERAPY (2018)PMID：30119194Reactivity：MouseSample Type：Serum,Tissue homogenate -
Aspergillus fumigatus Increased PAR-2 Expression and Elevated Proinflammatory Cytokines Expression Through the Pathway of PAR-2/ERK1/2 in Cornea
IF: 3.388Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2018)PMID：29332129Reactivity：MouseSample Type：Tissue homogenate -
Portulaca Extract Attenuates Development of Dextran Sulfate Sodium Induced Colitis in Mice through Activation of PPARγ
IF: 3.386 -
MicroRNA-27a alleviates LPS-induced acute lung injury in mice via inhibiting in?ammation and apoptosis through modulating TLR4/MyD88/NF-κB pathway
IF: 3.304Journal： CELL CYCLE (2018)PMID：30231673Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
Interaction of mannose binding lectin and other pattern recognition receptors in human corneal epithelial cells during Aspergillus fumigatus infection
IF: 3.118Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2018)PMID：30096599Reactivity：MouseSample Type：Tissue homogenate -
B-cell-specific mammalian target of rapamycin complex 1 activation results in severe osteoarthritis in mice
IF: 3.118 -
Diosgenin glucoside protects against myocardial injury in diabetic mice by inhibiting RIP140 signaling
IF: 3.061Journal： American Journal of Translational Research (2018)DOI：PMID:30662624PMID：30662624Reactivity：MouseSample Type：serum,cardiac tissue
实验操作视频
