Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
MiR-590-3p Attenuates Acute Kidney Injury by Inhibiting Tumor Necrosis Factor Receptor-Associated Factor 6 in Septic Mice
IF: 2.884 -
miR-124/MCP-1 signaling pathway modulates the protective effect of itraconazole on acute kidney injury in a mouse model of disseminated candidiasis
IF: 2.784Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2018)PMID：29568906Reactivity：MouseSample Type：serum -
Upregulated TSG-6 Expression in ADSCs Inhibits the BV2 Microglia-Mediated Inflammatory Response
IF: 2.583 -
Propofol specifically suppresses IL-1β secretion but increases bacterial survival in Staphylococcus aureus-infected RAW264.7 cells
IF: 2.561 -
Gentiopicroside abrogates lipopolysaccharide-induced depressive-like behavior in mice through tryptophan-degrading pathway
IF: 2.441 -
Dioscin prevents LPS?induced acute lung injury through inhibiting the TLR4/MyD88 signaling pathway via upregulation of HSP70
IF: 1.922Journal： Molecular Medicine Reports (2018)PMID：29512786Reactivity：MouseSample Type：Serum,Tissue homogenate -
Oral supplementation with ursolic acid ameliorates sepsis-induced acute kidney injury in a mouse model by inhibiting oxidative stress and inflammatory responses
IF: 1.922 -
Embelin inhibits abdominal aortic aneurysm through decreasing IL?6?induced STAT3 and NF?κB inactivation
IF: 1.922Journal： Molecular Medicine Reports (2018)PMID：29956759Reactivity：MouseSample Type：Aortic tissues -
The association between inflammation, the microbiome and urethane-induced pulmonary adenocarcinoma Corrigendum in /10.3892/ol.2018.9063
IF: 1.664 -
NLRP3 inflammasome signaling as an early molecular response is negatively controlled by miR-186 in CFA-induced prosopalgia mice
IF: 1.492Journal： BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH (2018)PMID：30020320Reactivity：MouseSample Type：Serum,Tissue homogenate
实验操作视频
