Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Sodium Alginate Hydrogel Infusion of Bone Marrow Mesenchymal Stem Cell‐Derived Extracellular Vesicles and p38α Antagonistic Peptides in Myocardial Infarction Fibrosis Mitigation
IF: 5Journal： Journal of the American Heart Association (2025)Reactivity：MouseSample Type：serum -
Integration of Transcriptomics and Proteomics to Elucidate Inhibitory Effect and Mechanism of Antifungalmycin B from Marine Streptomyces hiroshimensis in Treating Talaromyces marneffei
IF: 4.9 -
Neuroprotective Effects of Cilomilast and Chlorogenic Acid Against Scopolamine-Induced Memory Deficits via Modulation of the cAMP/PKA–CREB–BDNF Pathway
IF: 4.9Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2025)Reactivity：MouseSample Type：brain tissue -
Hydrogen sulfide preserves the function of senescent endothelium through SIRT2 mediated inflammatory inhibition
IF: 4.9Journal： JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2025)Reactivity：MouseSample Type：plasma -
Nitro-oleic acid alleviates inflammation and fibrosis by regulating macrophage polarization and fibroblast activation to improve cardiac function in MI mice
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：cardiac tissue -
Glycyrrhiza uralensis Polysaccharide Modulates Characteristic Bacteria and Metabolites, Improving the Immune Function of Healthy Mice
IF: 4.8 -
The absence of IRG1 exacerbates bone loss in a mouse model of ovariectomy‐induced osteoporosis by increasing osteoclastogenesis through the potentiation of NLRP3 inflammasome activation
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：Bone morrow tissue -
Calycosin extracted from Astragali Radix reduces NETs formation to improve renal fibrosis via TLR4/NF-κB pathway
IF: 4.8Journal： JOURNAL OF ETHNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：serum -
Urolithin A alleviates NLRP3 inflammasome activation and pyroptosis by promoting microglial mitophagy following spinal cord injury
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：serum -
Loss of Urat1 exacerbates APAP-induced liver injury in mice
IF: 4.8
实验操作视频
