Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Histone lactylation protects against sevoflurane-induced cognitive impairment by regulating YTHDF3/PRDX3 mediated microglial pyroptosis in neonatal mice
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：hippocampal tissue,serum -
Inhibiting HMGB1/AGER/NF-κB pathway prevents pro-inflammatory microglia polarization and protect photoreceptors in retinitis pigmentosa
IF: 4.8 -
Sweroside ameliorates IMQ-induced psoriasiform inflammation by inhibiting NLRP3/Caspase-1 mediated IL-1β elevation
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：serum -
Fluorofenidone mitigates liver fibrosis through GSK-3β modulation and hepatocyte protection in a 3D tissue-engineered model
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：liver tissue -
Multi-omics approaches reveal the therapeutic mechanism of Naoxintong Capsule against ischemic stroke
IF: 4.8Journal： JOURNAL OF ETHNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：colon tissue,serum,brain tissue -
Exploring macrophage and nerve interaction in endometriosis-associated pain: the inductive role of IL-33
IF: 4.8Journal： INFLAMMATION RESEARCH (2025)Reactivity：MouseSample Type：serum,peritoneal fluid (PF) -
Ameliorative Effect of Banana Lectin in TNBS-Induced Colitis in C57BL/6 Mice Relies on the Promotion of Antioxidative Mechanisms in the Colon
IF: 4.8 -
Fermented guava (Psidium guajava) by Lactiplantibacillus plantarum NCU0011129 attenuates azoxymethane/dextran sodium sulfate-induced colorectal cancer in mice: Restructuring gut microbiota and enhancing intestinal barrier function
IF: 4.8Journal： Food Bioscience (2025)Reactivity：MouseSample Type：Colonic tissue -
The emerging role of IL-22 as a potential radiosensitivity biomarker for radiation-induced intestinal injury
IF: 4.8Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)Reactivity：MouseSample Type：serum -
Parental high-fat diet induces upregulation of macrophage receptor with collagenous structure expression and exacerbates colorectal inflammation via the nuclear factor kappa-B pathway in offspring
IF: 4.8Journal： JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2025)Reactivity：MouseSample Type：serum
实验操作视频
