Mouse IL-2(Interleukin 2) ELISA Kit
中文名称：小鼠白介素2(IL-2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-2抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-2会与包被抗体结合。后依次加入生物素化的抗小鼠IL-2抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-2抗体与结合在包被抗体上的小鼠IL-2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D?deficient mice
IF: 1.851 -
Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress
IF: 1.751Journal： RESEARCH IN VETERINARY SCIENCE (2019)PMID：31412308Reactivity：MouseSample Type：Serum,Tissue homogenate -
Structural characterization of two endopolysaccharides from Phellinus sp. and their immunologic effects by intragastric administration in a healthy mammalian model
IF: 3.289 -
A trivalent Apx-fusion protein delivered by E. coli outer membrane vesicles induce protection against Actinobacillus pleuropneumoniae of serotype 1 and 7 challenge in a murine model
IF: 2.766 -
Rheb1 deletion in myeloid cells aggravates OVA-induced allergic inflammation in mice
IF: 4.259Journal： Scientific Reports (2017)PMID：28225024Reactivity：MouseSample Type：Bronchoalveolar Lavage Fluid,Serum -
Antitumor and immunomodulatory effects of ginsenoside Rh2 and its octyl ester derivative in H22 tumor-bearing mice
IF: 3.144Journal： Journal of Functional Foods (2017)Reactivity：MouseSample Type：Serum -
Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model
IF: 2.662 -
Immunogenicity of the recombinant HxuCBA proteins encoded by hxuCBA gene cluster of Haemophilus parasuis in mice
IF: 2.319 -
Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis
IF: 4.074Journal： CARBOHYDRATE POLYMERS (2015)PMID：26876821Reactivity：MouseSample Type：Cell culture supernatant -
Sulfated modification promotes the immunomodulatory bioactivities of Lyciumbarbarum polysaccharides in vitro
IF: 1.277Journal： International Journal of Clinical and Experimental Medicine (2015)DOI：PMID:26884954PMID：26884954Reactivity：Mouse
实验操作视频
