Mouse IL-33(Interleukin 33) ELISA Kit
中文名称：小鼠白介素33(IL-33)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-33抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-33会与包被抗体结合。后依次加入生物素化的抗小鼠IL-33抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-33抗体与结合在包被抗体上的小鼠IL-33结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-33浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-33的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-33，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Partial reduction of interleukin-33 signaling improves senescence and renal injury in diabetic nephropathy
IF: 10.700 -
Exposure to toluene diisocyanate induces dysbiosis of gut-lung homeostasis: Involvement of gut microbiota
IF: 7.600Journal： ENVIRONMENTAL POLLUTION (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
Interferon regulatory factor 7 alleviates the experimental colitis through enhancing IL-28A-mediated intestinal epithelial integrity
IF: 6.100Journal： Journal of Translational Medicine (2024)Reactivity：MouseSample Type：colonic tissue -
Mechanism of action of miR-15b-5p in alleviating asthma airway remodeling through the HMGB1/TLR4/IL-33 signaling axis
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
IL-33 triggers lung autophagy in anaphylaxis mice models
IF: 1.100Journal： Journal of Basic and Applied Zoology (2024)Reactivity：MouseSample Type：lung tissue -
Macrophage-derived inflammation promotes pulmonary vascular remodeling in hypoxia-induced pulmonary arterial hypertension mice
IF: 4.400 -
The STAT6 inhibitor AS1517499 reduces the risk of asthma in mice with 2,4-dinitrochlorobenzene-induced atopic dermatitis by blocking the STAT6 signaling pathway
IF: 3.373Journal： Allergy Asthma and Clinical Immunology (2022)PMID：35177102Reactivity：MouseSample Type：serum -
Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice
IF: 5.924 -
Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice
IF: 5.719 -
Remote ischemic post?conditioning alleviates ischemia/reperfusion?induced intestinal injury via the ERK signaling pathway?mediated RAGE/HMGB axis
IF: 2.952
实验操作视频
