Mouse IL-4(Interleukin 4) ELISA Kit
中文名称：小鼠白介素4(IL-4)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-4抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-4会与包被抗体结合。后依次加入生物素化的抗小鼠IL-4抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-4抗体与结合在包被抗体上的小鼠IL-4结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-4浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-4的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-4，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway
IF: 4.872Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020)PMID：32213367Reactivity：MouseSample Type：Cell culture supernatant -
Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function
IF: 4.546 -
Dabigatran ameliorates airway smooth muscle remodeling in asthma by modulating Yes-associated protein
IF: 4.486Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020)PMID：32542982Reactivity：MouseSample Type：Tissue homogenate -
MG53 Protects hUC-MSCs against Inflammatory Damage and Synergistically Enhances Their Efficacy in Neuroinflammation Injured Brain through Inhibiting NLRP3/Caspase-1/IL-1β Axis
IF: 4.486 -
Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin
IF: 4.192Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020)PMID：32182051Reactivity：Human,MouseSample Type：Serum,Cell culture supernatant -
A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC
IF: 4.187Journal： Microbial Cell Factories (2020)PMID：32075660Reactivity：MouseSample Type：Cell culture supernatant -
Ovalbumin causes impairment of preimplantation embryonic growth in asthma-induced mice
IF: 4.018 -
Myricitrin pretreatment ameliorates mouse liver ischemia reperfusion injury
IF: 3.943 -
Borneol inhibits CD4?+?T cells proliferation by down-regulating miR-26a and miR-142-3p to attenuate asthma
IF: 3.943Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)PMID：33272847Reactivity：mouseSample Type：Bronchoalveolar Lavage Fluid -
DHCR24 overexpression modulates microglia polarization and inflammatory response via Akt/GSK3β signaling in Aβ25–35 treated BV-2 cells
IF: 3.647
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