Mouse IL-4(Interleukin 4) ELISA Kit
中文名称：小鼠白介素4(IL-4)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-4抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-4会与包被抗体结合。后依次加入生物素化的抗小鼠IL-4抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-4抗体与结合在包被抗体上的小鼠IL-4结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-4浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-4的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-4，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Exposure time determines the protective effect of Trichinella spiralis on experimental colitis
IF: 2.914 -
Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection
IF: 2.914Journal： MICROBIAL PATHOGENESIS (2020)PMID：32781100Reactivity：MouseSample Type：Serum,Cell culture supernatant -
Colonisation of mice and pigs by a chimeric porcine circovirus 1–2 prototype vaccine strain and a PCV2 isolate originating in China and their induction of cytokines
IF: 1.786Journal： JOURNAL OF VIROLOGICAL METHODS (2020)PMID：32502500Reactivity：MouseSample Type：Cell culture supernatant -
Effects of programmed cell death protein 10 on fecundity in Schistosoma japonicum
IF: 1.641 -
Maternal 1-nitropyrene exposure during pregnancy increases susceptibility of allergic asthma in adolescent offspring
IF: 5.108 -
Severe Fever With Thrombocytopenia Syndrome Virus-Induced Macrophage Differentiation Is Regulated by miR-146
IF: 4.716 -
Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota
IF: 2.581Journal： MICROBIAL PATHOGENESIS (2019)PMID：31153984Reactivity：MouseSample Type：serum,small intestinal tissues -
Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice
IF: 2.482 -
BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D?deficient mice
IF: 1.851 -
Blocking PI3K/AKT signaling inhibits bone sclerosis in subchondral bone and attenuates post-traumatic osteoarthritis
IF: 3.923
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