Mouse IL-5(Interleukin 5) ELISA Kit
中文名称：小鼠白介素5(IL-5)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-5抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-5会与包被抗体结合。后依次加入生物素化的抗小鼠IL-5抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-5抗体与结合在包被抗体上的小鼠IL-5结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-5浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-5的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-5，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Exposure to toluene diisocyanate induces dysbiosis of gut-lung homeostasis: Involvement of gut microbiota
IF: 7.600Journal： ENVIRONMENTAL POLLUTION (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
CC16 alleviates PM2.5-induced lung epithelial cell injury and airway inflammation in asthmatic mice by inhibiting ferroptosis
IF: 6.200Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
The protective effects of Arctiin in asthma by attenuating airway inflammation and inhibiting p38/NF-κB signaling
IF: 5.200 -
Trim31 deficiency exacerbates airway inflammation in asthma by enhancing the activation of the NLRP3 inflammasome
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
Deletion of BRCC3 ameliorates airway inflammation in asthma by inhibiting the activation of NLRP3 inflammasome
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
Mechanism of action of miR-15b-5p in alleviating asthma airway remodeling through the HMGB1/TLR4/IL-33 signaling axis
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
CTSS contributes to airway neutrophilic inflammation in mixed granulocytic asthma
IF: 4.700Journal： RESPIRATORY RESEARCH (2024)Reactivity：MouseSample Type：Bronchoalveolar lavage fluid (BALF) -
Fibroblast Growth Factor 21 Confers Protection Against Asthma Through Inhibition of NLRP3 Inflammasome Activation
IF: 4.500Journal： INFLAMMATION (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
Listeria-vectored cervical cancer vaccine candidate strains reduce MDSCs via the JAK-STAT signaling pathway
IF: 4.400Journal： BMC BIOLOGY (2024)Reactivity：MouseSample Type：spleen tissue -
Sophora davidii Hance leaves total alkaloids (SDLTAs) alleviate asthma through inhibiting airway inflammation and regulating TLR4/MyD88/c-Jun pathway based on systematic pharmacology and molecular docking
IF: 3.800Journal： Journal of Functional Foods (2024)Reactivity：MouseSample Type：Alveolar lavage fluid (BALF)
实验操作视频
