Mouse IL-6(Interleukin 6) ELISA Kit
中文名称：小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
YOD1 sustains NOD2-mediated protective signaling in colitis by stabilizing RIPK2
IF: 6.500 -
Vangl2 suppresses NF-κB signaling and ameliorates sepsis by targeting p65 for NDP52-mediated autophagic degradation
IF: 6.400 -
Early biomarkers in the presymptomatic phase of cognitive impairment: changes in the endocannabinoidome and serotonergic pathways in Alzheimer's-prone mice after mTBI
IF: 6.200Journal： Acta Neuropathologica Communications (2024)Reactivity：MouseSample Type：plasma -
GAMG ameliorates silica-induced pulmonary inflammation and fibrosis via the regulation of EMT and NLRP3/TGF-β1/Smad signaling pathway
IF: 6.200Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
A Pectic Polysaccharide from Lycium ruthenicum Murray Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice
IF: 6.200Journal： Current Research in Food Science (2024)Reactivity：MouseSample Type：serum,colon tissue -
The combination of berberine and isoliquiritigenin synergistically improved adipose inflammation and obesity-induced insulin resistance
IF: 6.100 -
Atox1 regulates macrophage polarization in intestinal inflammation via ROS-NLRP3 inflammasome pathway
IF: 6.100Journal： Journal of Translational Medicine (2024)Reactivity：MouseSample Type：colon tissue -
Gut microbiota-derived acetate attenuates lung injury induced by influenza infection via protecting airway tight junctions
IF: 6.100Journal： Journal of Translational Medicine (2024)Reactivity：MouseSample Type：serum,Bronchoalveolar lavage fluid (BALF) -
HMGB1/TREM2 positive feedback loop drives the development of radioresistance and immune escape of glioblastoma by regulating TLR4/Akt signaling
IF: 6.100Journal： Journal of Translational Medicine (2024)Reactivity：MouseSample Type：serum -
Encapsulation of telmisartan inside insulinoma-cell-derived extracellular vesicles outperformed biomimetic nanovesicles in modulating the pancreatic inflammatory microenvironment
IF: 6.100Journal： Journal of Materials Chemistry B (2024)Reactivity：MouseSample Type：serum
实验操作视频
