Mouse IL-6(Interleukin 6) ELISA Kit
中文名称：小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Huanshaodan regulates microglial glucose metabolism reprogramming to alleviate neuroinflammation in AD mice through mTOR/HIF-1α signaling pathway
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：MouseSample Type：hippocampus tissue,cortex tissue -
Pharmacological mechanisms of Ma Xing Shi Gan Decoction in treating influenza virus-induced pneumonia: intestinal microbiota and pulmonary glycolysis
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：MouseSample Type：Serum,Lung tissue -
Thonningianin A ameliorated renal interstitial fibrosis in diabetic nephropathy mice by modulating gut microbiota dysbiosis and repressing inflammation
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：MouseSample Type：renal tissue -
Wedelolactone Mitigates Alcoholic Steatohepatitis via Modulating the TLR4/MyD88/NF-κB Pathway
IF: 4.400Journal： MEDIATORS OF INFLAMMATION (2024)Reactivity：MouseSample Type：serum -
Carvacrol alleviates LPS-induced myocardial dysfunction by inhibiting the TLR4/MyD88/NF-κB and NLRP3 inflammasome in cardiomyocytes
IF: 4.400Journal： Journal of Inflammation-London (2024)Reactivity：MouseSample Type：serum -
Effect of tangeretin on cisplatin-induced oxido-inflammatory brain damage in rats
IF: 4.300Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2024)Reactivity：MouseSample Type：brain tissue -
Kaempferol Alleviates Hepatic Injury in Nonalcoholic Steatohepatitis (NASH) by Suppressing Neutrophil-Mediated NLRP3-ASC/TMS1-Caspase 3 Signaling
IF: 4.200 -
Follicular CD8+ T cells promote immunoglobulin production and demyelination in multiple sclerosis and a murine model
IF: 4.200Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2024)Reactivity：MouseSample Type：serum -
FoxO1-modulated macrophage polarization regulates osteogenesis via PPAR-γ signaling
IF: 4.200Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2024)Reactivity：Mouse -
Protopanaxadiol Derivative: A Plant Origin of Novel Selective Glucocorticoid Receptor Modulator with Anti-inflammatory Effect
IF: 4.200
实验操作视频
