Mouse IL-6(Interleukin 6) ELISA Kit
中文名称：小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Preserved egg white alleviates DSS-induced colitis in mice through the reduction of oxidative stress, modulation of infl ammatory cytokines, NF-κB, MAPK and gut microbiota composition
IF: 8.022Journal： Food Science and Human Wellness (2022)Reactivity：MouseSample Type：colon tissue -
Irradiation-Induced Intestinal Injury is Associated With Disorders of Bile Acids Metabolism
IF: 8.013Journal： INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS (2022)Reactivity：MouseSample Type：intestine tissue -
The protective effects of sulforaphane on high-fat diet-induced metabolic associated fatty liver disease in mice via mediating the FXR/LXRα pathway
IF: 6.317Journal： Food & Function (2022)Reactivity：MouseSample Type：liver tissue,Serum -
Ultra-small molybdenum-based nanodots as an antioxidant platform for effective treatment of periodontal disease
IF: 6.064Journal： Frontiers in Bioengineering and Biotechnology (2022)Reactivity：MouseSample Type：gingival tissue -
Calycosin attenuates renal ischemia/reperfusion injury by suppressing NF-κB mediated inflammation via PPARγ/EGR1 pathway
IF: 5.988Journal： Frontiers in Pharmacology (2022)Reactivity：MouseSample Type：serum -
Epigallocatechin-3-gallate Mo nanoparticles (EGM NPs) efficiently treat liver injury by strongly reducing oxidative stress, inflammation and endoplasmic reticulum stress
IF: 5.988Journal： Frontiers in Pharmacology (2022)Reactivity：MouseSample Type：hepatic homogenates -
Network pharmacology-based and experimental identification of the effects of Renshen Yangrong decoction on myocardial infarction.
IF: 5.988Journal： Frontiers in Pharmacology (2022)Reactivity：MouseSample Type：serum -
Flos Puerariae-Semen Hoveniae medicinal pair extract ameliorates DSS-induced inflammatory bowel disease through regulating MAPK signaling and modulating gut microbiota composition
IF: 5.988Journal： Frontiers in Pharmacology (2022)Reactivity：MouseSample Type：colon tissue -
Nicotinamide N-methyltransferase ameliorates renal fibrosis by its metabolite 1-methylnicotinamide inhibiting the TGF-β1/Smad3 pathway
IF: 5.834Journal： FASEB JOURNAL (2022)Reactivity：MouseSample Type：kidney tissue -
ChK1 activation induces reactive astrogliosis through CIP2A/PP2A/STAT3 pathway in Alzheimer's disease
IF: 5.834
实验操作视频
