Mouse IL-6(Interleukin 6) ELISA Kit
中文名称：小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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3α-Angeloyloxy-ent-kaur-16-en-19-oic Acid Isolated from Wedelia trilobata L. Alleviates Xylene-Induced Mouse Ear Edema and Inhibits NF-κB and MAPK Pathway in LPS-Stimulated Macrophages
IF: 3.782Journal： JOURNAL OF NATURAL PRODUCTS (2020)PMID：33306388Reactivity：mouseSample Type：Cell culture medium,Tissue Slice -
Pretreatment of exosomes derived from hUCMSCs with TNF-α ameliorates acute liver failure by inhibiting the activation of NLRP3 in macrophage
IF: 3.647Journal： LIFE SCIENCES (2020)PMID：32035931Reactivity：MouseSample Type：Whole Blood,Tissue homogenate -
Camellia cake extracts reduce burn injury through suppressing inflammatory responses and enhancing collagen synthesis
IF: 3.647Journal： Food & Nutrition Research (2020)PMID：32425739Reactivity：MouseSample Type：Tissue homogenate -
Fenugreek attenuates obesity-induced inflammation and improves insulin resistance through downregulation of iRhom2/TACE
IF: 3.647 -
Effects of taraxasterol against ethanol and high-fat diet-induced liver injury by regulating TLR4/MyD88/NF-κB and Nrf2/HO-1 signaling pathways
IF: 3.647 -
The Protective Effects of Melatonin Against LPS-Induced Septic Myocardial Injury: A Potential Role of AMPK-Mediated Autophagy
IF: 3.644 -
Protective roles and mechanisms of rosmarinic acid in cyclophosphamide-induced premature ovarian failure
IF: 3.606Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2020)PMID：32711407Reactivity：MouseSample Type：Serum -
Methamphetamine induced neuroinflammation in mouse brain and microglial cell line BV2: Roles of the TLR4/TRIF/Peli1 signaling axis
IF: 3.569Journal： TOXICOLOGY LETTERS (2020)PMID：32768653Reactivity：MouseSample Type：Cell culture supernatant,Tissue homogenate -
Antineuroinflammatory Effects of 7,3’,4’-Trihydroxyisoflavone in Lipopolysaccharide-Stimulated BV2 Microglial Cells through MAPK and NF-κB Signaling Suppression
IF: 3.470Journal： Biomolecules & Therapeutics (2020)PMID：32812529Reactivity：MouseSample Type：Cell culture supernatant -
Development, optimisation and evaluation of chitosan nanoparticles of alendronate against Alzheimer’s disease in intracerebroventricular streptozotocin model for brain delivery
IF: 3.380Journal： JOURNAL OF DRUG TARGETING (2020)PMID：32876502Reactivity：MouseSample Type：Tissue homogenate
实验操作视频
