Mouse IL-6(Interleukin 6) ELISA Kit
中文名称：小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The Effects of RKI-1447 in a Mouse Model of Nonalcoholic Fatty Liver Disease Induced by a High-Fat Diet and in HepG2 Human Hepatocellular Carcinoma Cells Treated with Oleic Acid
IF: 1.918Journal： MEDICAL SCIENCE MONITOR (2020)PMID：32026851Reactivity：MouseSample Type：Serum,Cell culture supernatant,Tissue homogenate -
Chemerin-9 Peptide Enhances Memory and Ameliorates Aβ1–42-Induced Object Memory Impairment in Mice
IF: 1.863Journal： BIOLOGICAL & PHARMACEUTICAL BULLETIN (2020)PMID：31748466Reactivity：MouseSample Type：hippocampus -
Antioxidant and Anti-Inflammatory Activities of Kigelia africana (Lam.) Benth.
IF: 1.813Journal： Evidence-based Complementary and Alternative Medicine (2020)PMID：32655661Reactivity：Mouse -
Subcutaneous administration of casein attenuates atherosclerotic progression in male apoE-/- mice fed with high-fat diet
IF: 1.797 -
Clostridium butyricum protects intestinal barrier function via upregulation of tight junction proteins and activation of the Akt/mTOR signaling pathway in a mouse model of dextran sodium sulfate?induced colitis
IF: 1.785Journal： Experimental and Therapeutic Medicine (2020)PMID：32934675Reactivity：MouseSample Type：Serum -
Geniposide attenuates dextran sulfate sodium-induced colitis in mice via Nrf-2/HO-1/NF-κB pathway
IF: 1.681Journal： Annals of Palliative Medicine (2020)Reactivity：MouseSample Type：serum,colon tissue -
Role of Interleukin 17A in Aortic Valve Inflammation in Apolipoprotein E-deficient Mice.
IF: 1.273 -
Human umbilical cord mesenchymal stem cells suppress lung cancer via TLR4/NF-κB signalling pathway
IF: 1.186Journal： BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT (2020)Reactivity：MouseSample Type：Lewis cell-xenograft -
The TrkB agonist 7,8-dihydroxyflavone improves sensory-motor performance and reduces lipid peroxidation in old mice.
IF: 1.070 -
Antihypernociceptive, Anxiolytic, and Antidepressant Properties of Aqueous and Ethanol Extracts of Dissotis thollonii Cogn. (Melastomataceae) in Mice
Journal： Advances in Pharmacological and Pharmaceutical Sciences (2020)PMID：33015629Reactivity：MouseSample Type：Serum,Tissue homogenate
实验操作视频
