Mouse IL-6(Interleukin 6) ELISA Kit
中文名称：小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties
IF: 3.772 -
Inhibition of the mitochondrial complex-1 protects against carbon tetrachloride-induced acute liver injury
IF: 3.743 -
Inhibition of HDAC6 attenuates LPS-induced inflammation in macrophages by regulating oxidative stress and suppressing the TLR4-MAPK/NF-κB pathways
IF: 3.743Journal： BIOMEDICINE & PHARMACOTHERAPY (2019)PMID：31255993Reactivity：MouseSample Type：Cell culture supernatant -
Combination of tanshinone IIA and astragaloside IV attenuate atherosclerotic plaque vulnerability in ApoE(-/-) mice by activating PI3K/AKT signaling and suppressing TRL4/NF-κB signaling
IF: 3.743 -
Corilagin Interferes With Toll-Like Receptor 3-Mediated Immune Response in Herpes Simplex Encephalitis
IF: 3.720Journal： Frontiers in Molecular Neuroscience (2019)PMID：31080403Reactivity：MouseSample Type：brain tissues -
4-Methylcoumarin-[5,6–g]-hesperetin attenuates inflammatory responses in alcoholic hepatitis through PPAR-γ activation
IF: 3.547Journal： TOXICOLOGY (2019)PMID：30951781Reactivity：MouseSample Type：Serum,Cell culture supernatant -
Attractylone attenuates sepsis-associated encephalopathy and cognitive dysfunction by inhibiting microglial activation and neuroinflammation
IF: 3.448Journal： JOURNAL OF CELLULAR BIOCHEMISTRY (2019)PMID：30672013Reactivity：MouseSample Type：Cell culture supernatant,Tissue homogenate -
The protective effect of Geniposide on diabetic cognitive impairment through BTK/TLR4/NF-κB pathway
IF: 3.424 -
Pregnane X receptor activation by its natural ligand Ginkgolide-A improves tight junction proteins expression and attenuates bacterial translocation in cirrhosis
IF: 3.407Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2019)PMID：31697926Reactivity：MouseSample Type：Plasma,Tissue homogenate -
Tetrahydroxy stilbene glucoside alleviates palmitic acid-induced inflammation and apoptosis in cardiomyocytes by regulating miR-129-3p/Smad3 signaling
IF: 3.367Journal： CELLULAR & MOLECULAR BIOLOGY LETTERS (2019)PMID：30820195Reactivity：MouseSample Type：Cell culture supernatant
实验操作视频
