Mouse IL-6(Interleukin 6) ELISA Kit
中文名称：小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Role of Brd4 in the production of inflammatory cytokines in mouse macrophages treated with titanium particles
IF: 2.041Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2019)PMID：31330113Reactivity：MouseSample Type：Cell culture supernatant -
Anti-inflammatory effect of preserved egg with simulated gastrointestinal digestion on LPS-stimulated RAW264.7 cells
IF: 2.027 -
Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice
IF: 1.917Journal： BIOMEDICAL AND ENVIRONMENTAL SCIENCES (2019)PMID：30423277Reactivity：MouseSample Type：Plasma,Tissue homogenate -
Synergistic protection of matrine and lycopene against lipopolysaccharide?induced acute lung injury in mice
IF: 1.851 -
Kisspeptin?13 inhibits bleomycin?induced pulmonary fibrosis through GPR54 in mice
IF: 1.851 -
BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D?deficient mice
IF: 1.851 -
Alpinia officinarum Hance extract alleviates particulate matter-induced lung injury in mice
IF: 1.772Journal： Asian Pacific Journal of Tropical Medicine (2019)Reactivity：MouseSample Type：lung tisssue -
Notch signalling regulates steroidogenesis in mouse ovarian granulosa cells
IF: 1.723Journal： REPRODUCTION FERTILITY AND DEVELOPMENT (2019)DOI：10.1071/RD18281PMID：30827331Reactivity：MouseSample Type：Cell culture medium -
The therapeutic effects of resveratrol on hepatic steatosis in high-fat diet-induced obese mice by improving oxidative stress, inflammation and lipid-related gene transcriptional expression
IF: 1.383Journal： Medical Molecular Morphology (2019)PMID：30673851Reactivity：MouseSample Type：Serum,Tissue homogenate -
Antioxidant and anti-inflammatory effects of an ethanol fraction from the Schisandra chinensis baillon hot water extract fermented using Lactobacilius paracasei subsp. tolerans
IF: 0.888
实验操作视频
