Mouse IL-6(Interleukin 6) ELISA Kit
中文名称：小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Osthole prevents acetaminophen-induced liver injury in mice
IF: 3.223Journal： ACTA PHARMACOLOGICA SINICA (2017)PMID：29022574Reactivity：MouseSample Type：Tissue homogenate -
Wogonin attenuates inflammation by activating PPAR-γ in alcoholic liver disease
IF: 2.956Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2017)PMID：28646664Reactivity：MouseSample Type：Serum,Cell culture supernatant -
Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic Cells
IF: 2.861 -
Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet
IF: 2.806 -
Therapeutic effect of imiquimod on dextran sulfate sodium-induced ulcerative colitis in mice
IF: 2.806 -
Bone marrow mesenchymal stem cells accelerate the hyperglycemic refractory wound healing by inhibiting an excessive inflammatory response
IF: 1.692 -
Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice
IF: 4.492Journal： Oxidative Medicine and Cellular Longevity (2016)PMID：27413418Reactivity：MouseSample Type：Plasma -
Effects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii
IF: 4.320Journal： International Journal of Nanomedicine (2016)PMID：27574420Reactivity：MouseSample Type：Plasma -
Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis
IF: 4.074Journal： CARBOHYDRATE POLYMERS (2015)PMID：26876821Reactivity：MouseSample Type：Cell culture supernatant -
PGC-1? silencing compounds the perturbation of mitochondrial function caused by mutant SOD1 in skeletal muscle of ALS mouse model
IF: 4.000
实验操作视频
