Mouse IL-6(Interleukin 6) ELISA Kit
中文名称：小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Hydrogen sulfide attenuates oxidative stress-induced cellular senescence via the Sirt3/SOD2 signaling pathway in chronic obstructive pulmonary disease
IF: 7.5Journal： CHINESE MEDICAL JOURNAL (2025)Reactivity：MouseSample Type：lung tissue -
Astrocytic spermidine insufficiency contributes to enhanced pain sensitivity associated with ApoE4
IF: 7.3Journal： JOURNAL OF HEADACHE AND PAIN (2025)Reactivity：MouseSample Type：spinal dorsal horn tissue -
Integration of Multi-Omics Data and Machine Learning to Identify Antioxidant Biomarkers in Type 1 Diabetes
IF: 7.1Journal： FREE RADICAL BIOLOGY AND MEDICINE (2025)Reactivity：MouseSample Type：serum -
Puerarin relives inflammation, bone destruction and facilitates osteogenic differentiation in periodontitis by enhancing mitochondrial autophagy via activating mitochondrial Mitofusin 2
IF: 7.1Journal： Stem Cell Research & Therapy (2025)Reactivity：MouseSample Type：periapical tissue -
Akebia trifoliata extracts attenuate liver injury via gut–liver axis in a murine model of nonalcoholic fatty liver disease with low-grade colitis
IF: 7.0Journal： FOOD RESEARCH INTERNATIONAL (2025)Reactivity：MouseSample Type：liver tissue,serum -
Cyclic di AMP phosphodiesterase nanovaccine elicits protective immunity against Burkholderia cenocepacia infection in mice
IF: 6.9 -
Control of aggressive 4T1-luc metastatic breast cancer using immunogenic cell lysates generated with methotrexate
IF: 6.9Journal： BIOMEDICINE & PHARMACOTHERAPY (2025)Reactivity：MouseSample Type：serum -
Pregnane X receptor alleviates sepsis-induced liver injury through activation of yes-associated protein in mice
IF: 6.9Journal： ACTA PHARMACOLOGICA SINICA (2025)Reactivity：MouseSample Type：serum,liver tissue -
Design and Synthesis of Hederagenin Derivatives for the Treatment of Sepsis by Targeting TAK1 and Regulating the TAK1-NF-κB/MAPK Signaling
IF: 6.8Journal： JOURNAL OF MEDICINAL CHEMISTRY (2025)Reactivity：MouseSample Type：serum -
Ginsenoside Rh2 Alleviates Inflammatory Response and Enhances Motor Function Recovery in Spinal Cord Injury Mice via the ROS/MAPK14 Signaling Pathway
IF: 6.8
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