Mouse IL-6(Interleukin 6) ELISA Kit
中文名称：小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素，抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Diosgenin glucoside protects against myocardial injury in diabetic mice by inhibiting RIP140 signaling
IF: 3.061Journal： American Journal of Translational Research (2018)DOI：PMID:30662624PMID：30662624Reactivity：MouseSample Type：serum,cardiac tissue -
Protective effects of SKLB023 on a mouse model of unilateral ureteral obstruction by the modulation of gut microbiota
IF: 2.936 -
Maggot protein ameliorates dextran sulphate sodium-induced ulcerative colitis in mice
IF: 2.899 -
MiR-590-3p Attenuates Acute Kidney Injury by Inhibiting Tumor Necrosis Factor Receptor-Associated Factor 6 in Septic Mice
IF: 2.884 -
Resveratrol Protects Against Renal Damage via Attenuation of Inflammation and Oxidative Stress in High-Fat-Diet-Induced Obese Mice
IF: 2.884 -
miR-124/MCP-1 signaling pathway modulates the protective effect of itraconazole on acute kidney injury in a mouse model of disseminated candidiasis
IF: 2.784Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2018)PMID：29568906Reactivity：MouseSample Type：serum -
Upregulated TSG-6 Expression in ADSCs Inhibits the BV2 Microglia-Mediated Inflammatory Response
IF: 2.583 -
Eosinophils attenuate arthritis by inducing M2 macrophage polarization via inhibiting the IκB/P38 MAPK signaling pathway
IF: 2.559Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018)PMID：30528734Reactivity：MouseSample Type：Tissue homogenate,Cell culture supernatant -
Effect of gap junctions on RAW264.7 macrophages infected with H37Rv
IF: 2.028 -
Dioscin prevents LPS?induced acute lung injury through inhibiting the TLR4/MyD88 signaling pathway via upregulation of HSP70
IF: 1.922Journal： Molecular Medicine Reports (2018)PMID：29512786Reactivity：MouseSample Type：Serum,Tissue homogenate
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