Mouse INS(Insulin) ELISA Kit
中文名称：小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素，抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Acetate enables metabolic fitness and cognitive performance during sleep disruption
IF: 27.700 -
Beta-Cell Tipe1 Orchestrates Insulin Secretion and Cell Proliferation by Promoting Gαs/cAMP Signaling via USP5
IF: 15.100 -
rs-TAC PDC, a peptide drug-conjugate, for targeted delivery of tacrolimus and sericin alleviates podocyte injury in diabetic nephropathy
IF: 13.200Journal： Nano Today (2024)Reactivity：MouseSample Type：plasma,pancreas tissue -
Biomimetic nanoplatform with microbiome modulation and antioxidant functions ameliorating insulin resistance and pancreatic β-cell dysfunction for T2DM management
IF: 12.800Journal： BIOMATERIALS (2024)Reactivity：MouseSample Type：Serum -
Baicalein, a dietary flavonoid, enhances the insulin-sensitizing effect of metformin to prevent type 2 diabetes via the regulation of lipid metabolism and gut microenvironment
IF: 9.900 -
Accumulated BCAAs and BCKAs contribute to the HFD-induced deterioration of Alzheimer's disease via a dysfunctional TREM2-related reduction in microglial β-amyloid clearance
IF: 9.300Journal： Journal of Neuroinflammation (2024)Reactivity：MouseSample Type：serum -
The polysaccharides from seeds of Glycyrrhiza uralensis ameliorate metabolic disorders and restructure gut microbiota in type 2 diabetic mice
IF: 8.200Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)PMID：38447833Reactivity：MouseSample Type：serum -
PPARγ Agonist Pioglitazone Prevents Hypoxia-induced Cardiac Dysfunction by Reprogramming Glucose Metabolism
IF: 8.200Journal： International Journal of Biological Sciences (2024)Reactivity：MouseSample Type：Plasma -
Prebiotics modulate the microbiota–gut–brain axis and ameliorate anxiety and depression-like behavior in HFD-fed mice
IF: 8.100 -
Liver ChREBP deficiency inhibits fructose-induced insulin resistance in pregnant mice and female offspring
IF: 7.700
实验操作视频
