Mouse INS(Insulin) ELISA Kit
中文名称：小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素，抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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A decrease in cluster of differentiation 2 expression on natural killer cells is associated with polycystic ovary syndrome but not influenced by metformin in a mouse model
IF: 4.161 -
Sox9 is required in regeneration of pancreatic β cells following injury
IF: 4.145 -
Cadmium exposure decreases fasting blood glucose levels and exacerbates type-2 diabetes in a mouse model
IF: 3.925 -
Comparative study on physicochemical characteristics, α-glucosidase inhibitory effect, and hypoglycemic activity of pectins from normal and Huanglongbing-infected navel orange peels
IF: 3.654 -
Metabolomics study on liver of db/db mice treated with curcumin using UPLC-Q-TOF-MS
IF: 3.571Journal： JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2022)PMID：35461164Reactivity：MouseSample Type：plasma -
Sigma?1 receptor overexpression promotes proliferation and ameliorates cell apoptosis in β?cells
IF: 3.423 -
Salidroside Affects Gut Microbiota Structure in db/db Mice by Affecting Insulin, Blood Glucose and Body Weight
IF: 3.249Journal： Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2022)PMID：36060789Reactivity：MouseSample Type：serum -
Cistanche tubulosa phenylethanoid glycosides suppressed adipogenesis in?3T3-L1?adipocytes?and improved obesity and insulin resistance in high-fat diet induced obese mice
IF: 2.838Journal： BMC Complementary Medicine and Therapies (2022)PMID：36229811Reactivity：MouseSample Type：serum -
Direct Reprogramming of Mice Skin Fibroblasts into Insulin-Producing Cells In Vitro
IF: 2.257 -
Primaquine activates Keratin 7 to treat diabetes and its complications
Journal： Journal of Diabetes and Metabolic Disorders (2022)PMID：36404863Reactivity：MouseSample Type：liver tissue
实验操作视频
