Mouse INS(Insulin) ELISA Kit
中文名称：小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素，抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Sigma receptor knockdown augments dysfunction and apoptosis of beta cells induced by palmitate
IF: 2.691Journal： EXPERIMENTAL BIOLOGY AND MEDICINE (2021)PMID：33715527Reactivity：mouseSample Type：Cell culture supernatant -
Huang-Pu-Tong-Qiao Formula Ameliorates the Hippocampus Apoptosis in Diabetic Cognitive Dysfunction Mice by Activating CREB/BDNF/TrkB Signaling Pathway
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：34211563Reactivity：MouseSample Type：hippocampu -
Effect of Electroacupuncture on Reproductive Disorders and Insulin Resistance in a Murine Polycystic Ovary Syndrome Model
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：34987599Reactivity：MouseSample Type：serum -
Death-associated protein kinase 1 correlates with podocyte apoptosis and renal damage and can be mediated by miR-361.
IF: 2.303 -
Andrographolide Exerts Antihyperglycemic Effect through Strengthening Intestinal Barrier Function and Increasing Microbial Composition of Akkermansia muciniphila
IF: 5.076Journal： Oxidative Medicine and Cellular Longevity (2020)PMID：32774682Reactivity：MouseSample Type：Serum -
Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells
IF: 4.545 -
Modulation of Gut Microbiota by Fucoxanthin During Alleviation of Obesity in High-Fat Diet-Fed Mice
IF: 4.192Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020)PMID：32309947Reactivity：MouseSample Type：Serum,Tissue homogenate -
Intra-Pancreatic Insulin Nourishes Cancer Cells: Do Insulin-Receptor Antagonists such as PGG and EGCG Play a Role?
IF: 3.682Journal： AMERICAN JOURNAL OF CHINESE MEDICINE (2020)PMID：32468825Reactivity：MouseSample Type：Plasma -
Berberine attenuated olanzapine-induced metabolic alterations in mice: Targeting transient receptor potential vanilloid type 1 and 3 channels
IF: 3.647 -
Role of TRPV1/TRPV3 channels in olanzapine-induced metabolic alteration: Possible involvement in hypothalamic energy-sensing, appetite regulation, inflammation and mesolimbic pathway
IF: 3.347Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2020)PMID：32652086Reactivity：MouseSample Type：Serum
实验操作视频
