Mouse INS(Insulin) ELISA Kit
中文名称：小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素，抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Lpl-C310R mutation is associated with impaired glucose tolerance and endoplasmic reticulum stress in skeletal muscle
IF: 2.985Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2020)PMID：32703455Reactivity：MouseSample Type：Plasma -
Catechins from oolong tea improve uterine defects by inhibiting STAT3 signaling in polycystic ovary syndrome mice
IF: 2.960 -
A?aí seed extract prevents the renin-angiotensin system activation, oxidative stress and inflammation in white adipose tissue of high-fat diet–fed mice
IF: 2.767 -
GLP-1 preserves β cell function via improvement on islet insulin signaling in high fat diet feeding mice
IF: 2.411 -
β-Sitosterol Shows Potential to Protect Against the Development of High-Fructose Diet-Induced Metabolic Dysfunction in Female Rats
IF: 2.040 -
The protective effect of sulforaphane on type II diabetes induced by high-fat diet and low-dosage streptozotocin
IF: 1.797 -
Rotenone protects against β-cell apoptosis and attenuates type 1 diabetes mellitus
IF: 4.021 -
Therapeutic potential of NaoXinTong Capsule on the developed diabetic nephropathy in db/db mice
IF: 3.743Journal： BIOMEDICINE & PHARMACOTHERAPY (2019)PMID：31545275Reactivity：MouseSample Type：Serum,Urine -
Modeling of antipsychotic-induced metabolic alterations in mice: An experimental approach precluding psychosis as a predisposing factor
IF: 3.585Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2019)PMID：31254565Reactivity：MouseSample Type：Serum -
Pelargonidin-3-O-glucoside Derived from Wild Raspberry Exerts Antihyperglycemic Effect by Inducing Autophagy and Modulating Gut Microbiota
IF: 3.571Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2019)PMID：31322351Reactivity：MouseSample Type：Serum
实验操作视频
