Mouse INS(Insulin) ELISA Kit
中文名称：小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素，抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis
IF: 3.329 -
The therapeutic effects of resveratrol on hepatic steatosis in high-fat diet-induced obese mice by improving oxidative stress, inflammation and lipid-related gene transcriptional expression
IF: 1.383Journal： Medical Molecular Morphology (2019)PMID：30673851Reactivity：MouseSample Type：Serum,Tissue homogenate -
Role of O-GlcNAcylation and endoplasmic reticulum stress on obesity and insulin resistance
IF: 0.329Journal： Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2019)Reactivity：MouseSample Type：plasma -
Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines
IF: 12.354 -
Procyanidin B2 ameliorates free fatty acids-induced hepatic steatosis through regulating TFEB-mediated lysosomal pathway and redox state
IF: 6.020 -
Polysaccharides extracted from Phellinus linteus ameliorate high-fat high-fructose diet induced insulin resistance in mice
IF: 5.158 -
Baicalin regulates SirT1/STAT3 pathway and restrains excessive hepatic glucose production
IF: 4.897Journal： PHARMACOLOGICAL RESEARCH (2018)PMID：30144531Reactivity：MouseSample Type：Cell culture supernatant -
Effects of triphenyl phosphate exposure during fetal development on obesity and metabolic dysfunctions in adult mice: Impaired lipid metabolism and intestinal dysbiosis
IF: 4.358 -
NRF2 mitigates acute alcohol-induced hepatic and pancreatic injury in mice
IF: 3.977 -
Diet induced maternal obesity affects offspring gut microbiota and persists into young adulthood
IF: 3.289
实验操作视频
