Mouse INS(Insulin) ELISA Kit
中文名称：小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素，抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
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Accumulated BCAAs and BCKAs contribute to the HFD-induced deterioration of Alzheimer's disease via a dysfunctional TREM2-related reduction in microglial β-amyloid clearance
IF: 9.3Journal： Journal of Neuroinflammation (2024)Reactivity：MouseSample Type：serum -
The polysaccharides from seeds of Glycyrrhiza uralensis ameliorate metabolic disorders and restructure gut microbiota in type 2 diabetic mice
IF: 8.2Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)PMID：38447833Reactivity：MouseSample Type：serum -
PPARγ Agonist Pioglitazone Prevents Hypoxia-induced Cardiac Dysfunction by Reprogramming Glucose Metabolism
IF: 8.2Journal： International Journal of Biological Sciences (2024)Reactivity：MouseSample Type：Plasma -
Prebiotics modulate the microbiota–gut–brain axis and ameliorate anxiety and depression-like behavior in HFD-fed mice
IF: 8.1 -
Liver ChREBP deficiency inhibits fructose-induced insulin resistance in pregnant mice and female offspring
IF: 7.7 -
Effects of three Huanglian-derived polysaccharides on the gut microbiome and fecal metabolome of high-fat diet/streptozocin-induced type 2 diabetes mice
IF: 7.7Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)Reactivity：MouseSample Type：plasma -
Dendrobium officinale regulate lipid metabolism in diabetic mouse liver via PPAR-RXR signaling pathway: Evidence from an integrated multi-omics analysis
IF: 7.5 -
Gut microbiota dysbiosis and oxidative damage in high-fat diet-induced impairment of spermatogenesis: Role of protocatechuic acid intervention
IF: 7.4 -
Impact of vitamin A on the risk of mild cognitive impairment and Alzheimer's disease pathology in high-fat-diet–treated APP/PS1 mice
IF: 7.4 -
Lipid metabolic reprogramming mediated by circulating Nrg4 alleviates metabolic dysfunction-associated steatotic liver disease during the early recovery phase after sleeve gastrectomy
IF: 7.0
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