Mouse INS(Insulin) ELISA Kit
中文名称：小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素，抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Arsenic exposure accelerates type 1 diabetes mellitus progression via pyroptosis pathway in mice
IF: 4.700Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2024)Reactivity：MouseSample Type：serum -
Anorexigenic and anti-inflammatory signaling pathways of semaglutide via the microbiota–gut––brain axis in obese mice
IF: 4.600Journal： INFLAMMOPHARMACOLOGY (2024)Reactivity：MouseSample Type：Serum -
Effect of Semaglutide on High-Fat-Diet-Induced Liver Cancer in Obese Mice
IF: 4.400 -
Branched-chain amino acid supplementation drives dynamic changes in gut microbiota without impairing glucose and lipid homeostasis at the different stages of insulin resistance in mice on high-fat diet
IF: 4.400 -
Liraglutide improved the reproductive function of obese mice by upregulating the testicular AC3/cAMP/PKA pathway
IF: 4.400Journal： Reproductive Biology and Endocrinology (2024)PMID：38509558Reactivity：MouseSample Type：serum -
Effects of Extracellular Vesicles Derived from Human Umbilical Cord Blood Mesenchymal Stem Cells on Cell Immunity in Nonobese Mice
IF: 4.300 -
Intermedin Alleviates Diabetic Cardiomyopathy by Up-Regulating CPT-1β through Activation of the Phosphatidyl Inositol 3 Kinase/Protein Kinase B Signaling Pathway
IF: 4.300 -
Protective effects of menthol against olanzapine-induced metabolic alterations in female mice
IF: 4.200Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
Adipose tissue macrophage-derived microRNA-210-3p disrupts systemic insulin sensitivity by silencing GLUT4 in obesity
IF: 4.000 -
2-Hydroxylation is a chemical switch linking fatty acids to glucose-stimulated insulin secretion
IF: 4.000Journal： JOURNAL OF BIOLOGICAL CHEMISTRY (2024)Reactivity：MouseSample Type：serum
实验操作视频
