Mouse INS(Insulin) ELISA Kit
中文名称：小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素，抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Berberine ameliorates glucocorticoid-induced hyperglycemia: an in vitro and in vivo study
IF: 3.600Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023)PMID：37704773Reactivity：MouseSample Type：plasma -
Sulfathiazole treats type 2 diabetes by restoring metabolism through activating CYP19A1
IF: 3.000Journal： BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS (2023)PMID：36627088Reactivity：MouseSample Type：plasma -
TFP5 attenuates cyclin-dependent kinase 5-mediated islet β-cell damage in diabetes
IF: 3.000 -
Liraglutide Attenuates Aortic Valve Calcification in a High-Cholesterol-Diet-Induced Experimental Calcific Aortic Valve Disease Model in Apolipoprotein E-Deficient Mice
IF: 2.400Journal： Journal of Cardiovascular Development and Disease (2023)PMID：37754815Reactivity：MouseSample Type：Plasma -
Cholesterol induces inflammation and reduces glucose utilization
IF: 2.100 -
High expression of the glutathione S-transferase A2 and neuropilin-2 genes affects pancreatic islet β-cell function
IF: 0.900Journal： International Journal of Diabetes in Developing Countries (2023)Reactivity：Mouse -
Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease
IF: 17.694 -
Lipid-induced monokine cyclophilin-A promotes adipose tissue dysfunction implementing insulin resistance and type 2 diabetes in zebrafish and mice models of obesity
IF: 9.207Journal： CELLULAR AND MOLECULAR LIFE SCIENCES (2022)PMID：35511344Reactivity：MouseSample Type：plasma -
Chemerin-Induced Down-Regulation of Placenta-Derived Exosomal miR-140-3p and miR-574-3p Promotes Umbilical Vein Endothelial Cells Proliferation, Migration, and Tube Formation in Gestational Diabetes Mellitus
IF: 7.666 -
Effects of distinct n-6 to n-3 polyunsaturated fatty acid ratios on insulin resistant and AD-like phenotypes in high-fat diets-fed APP/PS1 mice
IF: 7.425Journal： FOOD RESEARCH INTERNATIONAL (2022)PMID：36461380Reactivity：MouseSample Type：serum, cortical
实验操作视频
