Mouse INS(Insulin) ELISA Kit
中文名称：小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素，抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 细胞培养上清、组织匀浆上清 |
| 特异性 | 可检测样本中的小鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Comparative analysis of fermented barley dietary fiber by Lactiplantibacillus plantarum dy-1 promoting gut health and regulating hepatic energy metabolism in high fat diet induced obese mice
IF: 5.100 -
Broccoli alleviates metabolic-associated fatty liver disease through regulating gut flora and lipid metabolism via the FXR/LXR signaling pathway
IF: 5.100Journal： Food & Function (2024)Reactivity：MouseSample Type：Serum,hepatic tissue -
Protective Effects of Isostrictiniin Against High-Fat, High-Sugar Diet-Induced Steatosis in MASLD Mice via Regulation of the AMPK/SREBP-1c/ACC Pathway
IF: 4.800 -
Relationship Between Metabolomics of T2DM Patients and the Anti-Diabetic Effects of Phellodendri Chinensis Cortex-Anemarrhenae Rhizoma Herb Pair in Mice
IF: 4.800Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum,pancreatic tissue -
Protective Effect of Modified Suanmei-Tang on Metabolic-Associated Fatty Liver Disease: An Integrated Strategy of Network Pharmacology, Metabolomics, and Transcriptomics
IF: 4.700Journal： Drug Design Development and Therapy (2024)Reactivity：MouseSample Type：serum -
Semaglutide Reduces Cardiomyocyte Damage Caused by High-Fat Through HSDL2
IF: 4.700Journal： Drug Design Development and Therapy (2024)Reactivity：MouseSample Type：plasma -
Arsenic exposure accelerates type 1 diabetes mellitus progression via pyroptosis pathway in mice
IF: 4.700Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2024)Reactivity：MouseSample Type：serum -
Evaluating the therapeutic potential of moxibustion on polycystic ovary syndrome: a rat model study on gut microbiota and metabolite interaction
IF: 4.600Journal： Frontiers in Cellular and Infection Microbiology (2024)Reactivity：RatSample Type：serum -
Anorexigenic and anti-inflammatory signaling pathways of semaglutide via the microbiota–gut––brain axis in obese mice
IF: 4.600Journal： INFLAMMOPHARMACOLOGY (2024)Reactivity：MouseSample Type：Serum -
2-Hydroxylation is a chemical switch linking fatty acids to glucose-stimulated insulin secretion
IF: 4.000Journal： JOURNAL OF BIOLOGICAL CHEMISTRY (2024)Reactivity：MouseSample Type：serum
实验操作视频
