Mouse INS(Insulin) ELISA Kit
中文名称：小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上，实验时样品（或标准品）中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素，抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 细胞培养上清、组织匀浆上清 |
| 特异性 | 可检测样本中的小鼠INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Proteomic analysis reveals semaglutide impacts lipogenic protein expression in epididymal adipose tissue of obese mice
IF: 5.200Phage cocktail alleviated type 2 diabetes by reshaping gut microbiota and decreasing proinflammatory cytokines
IF: 5.000Journal： APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2023)Reactivity：MouseSample Type：Blood sampleAntiobesity effect of L-arabinose via ameliorating insulin resistance and modulating gut microbiota in obese mice
IF: 4.400FUNDC1 modulates mitochondrial defects and pancreatic β-cell dysfunction under lipotoxicity
IF: 3.100PP2Ac knockdown attenuates lipotoxicity?induced pancreatic β?cell dysfunction and apoptosis
IF: 2.700Journal： Experimental and Therapeutic Medicine (2023)PMID：37928506Reactivity：MouseSample Type：serumEndoplasmic reticulum stress in the adipose tissue and monocyte chemoattractant protein-1 are involved in tacrolimus-induced diabetes mellitus
IF: 2.600Effects of semaglutide on vascular structure and proteomics in high-fat diet-induced obese mice
IF: 6.055Journal： Frontiers in Endocrinology (2022)Reactivity：MouseSample Type：plasmaDirect Reprogramming of Mice Skin Fibroblasts into Insulin-Producing Cells In Vitro
IF: 2.257Inhibition of Notch activity promotes pancreatic cytokeratin 5-positive cell differentiation to beta cells and improves glucose homeostasis following acute pancreatitis
IF: 8.469Ascorbic Acid and Alpha-Tocopherol Contribute to the Therapy of Polycystic Ovarian Syndrome in Mouse Models
IF: 2.616
