Mouse IP-10/CXCL10(Interferon Gamma Induced Protein 10kDa) ELISA Kit
中文名称：小鼠10kDa干扰素γ诱导蛋白(IP-10/CXCL10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IP-10/CXCL10抗体包被于酶标板上，实验时样品（或标准品）中的小鼠IP-10/CXCL10会与包被抗体结合。后依次加入生物素化的抗小鼠IP-10/CXCL10抗体和辣根过氧化物酶标记的亲和素，抗小鼠IP-10/CXCL10抗体与结合在包被抗体上的小鼠IP-10/CXCL10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IP-10/CXCL10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IP-10/CXCL10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠IP-10/CXCL10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Peroxidase-Like Nanozyme Activates the cGAS-STING Pathway via ROS-Induced mtDNA Release for Cancer Immunotherapy
IF: 18.500Journal： ADVANCED FUNCTIONAL MATERIALS (2024)Reactivity：MouseSample Type：MC38 cell-Xenograft -
Genetically Edited Cascade Nanozymes for Cancer Immunotherapy
IF: 15.800Journal： ACS Nano (2024)Reactivity：MouseSample Type：4T1 cell-Xenograft -
A hypoxia-activated and microenvironment-remodeling nanoplatform for multifunctional imaging and potentiated immunotherapy of cancer
IF: 14.700 -
Dihydroartemisinin Modulates Enteric Glial Cell Heterogeneity to Alleviate Colitis
IF: 14.300 -
Multi-Metallic Nanosheets Reshaping Immunosuppressive Tumor Microenvironment through Augmenting cGAS-STING Innate Activation and Adaptive Immune Responses for Cancer Immunotherapy
IF: 14.300Journal： Advanced Science (2024)Reactivity：MouseSample Type：4T1 cell-Xenograft -
Tumorous IRE1α facilitates CD8+T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma
IF: 8.400 -
CSF1R blockade slows progression of cerebral hemorrhage by reducing microglial proliferation and increasing infiltration of CD8?+?CD122+ T cells into the brain
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：brain tissue -
A novel oral TLR7 agonist orchestrates immune response and synergizes with PD-L1 blockade via type I IFN pathway in lung cancer
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：LLC cell-Xenograft -
Original research: Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis
IF: 12.469Journal： Journal for ImmunoTherapy of Cancer (2022)PMID：35738798Reactivity：MouseSample Type：B16F10 cell-xenograft -
Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism
IF: 10.723Journal： CARBOHYDRATE POLYMERS (2022)PMID：36184177Reactivity：MouseSample Type：bronchoalveolar fluid
实验操作视频
