Mouse Irisin ELISA Kit
中文名称：小鼠鸢尾素(Irisin)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 78.13-5000 pg/mL
- 灵敏度： 46.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠Irisin抗体包被于酶标板上，实验时样品（或标准品）中的小鼠Irisin会与包被抗体结合。后依次加入生物素化的抗小鼠Irisin抗体和辣根过氧化物酶标记的亲和素，抗小鼠Irisin抗体与结合在包被抗体上的小鼠Irisin结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Irisin浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Irisin的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 78.13-5000 pg/mL |
| 灵敏度 | 46.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠Irisin，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Irisin improves diabetic cardiomyopathy-induced cardiac remodeling by regulating GSDMD-mediated pyroptosis through MITOL/STING signaling
IF: 7.500 -
A Combination of Exercise and Yogurt Intake Protects Mice against Obesity by Synergistic Promotion of Adipose Browning
IF: 5.700Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：Plasma -
Irisin protects against cardiac injury by inhibiting NLRP3 inflammasome-mediated pyroptosis during remodeling after infarction
IF: 5.600 -
Docosahexaenoic and eicosapentaenoic acids promote the accumulation of browning-related myokines via calcium signaling in insulin-resistant mice
IF: 4.200 -
ELK4 ameliorates cognitive impairment and neuroinflammation induced by obstructive sleep apnea
IF: 3.500Journal： BRAIN RESEARCH BULLETIN (2024)Reactivity：MouseSample Type：Hippocampal tissue -
Irisin ameliorates UUO-induced renal interstitial fibrosis through TGF-β1/periostin/MMP-2 signaling pathway
IF: 2.900 -
Irisin Ameliorated Skeletal Muscle Atrophy by Inhibiting Fatty Acid Oxidation and Pyroptosis Induced by Palmitic Acid in Chronic Kidney Disease
IF: 2.800 -
Exercise-induced FNDC5/irisin protects nucleus pulposus cells against senescence and apoptosis by activating autophagy
IF: 12.153Journal： EXPERIMENTAL AND MOLECULAR MEDICINE (2022)PMID：35882943Reactivity：MouseSample Type：plasma -
Irisin protects against vascular calcification by activating autophagy and inhibiting NLRP3-mediated vascular smooth muscle cell pyroptosis in chronic kidney disease
IF: 9.685 -
Asprosin, a novel glucogenic adipokine: a potential therapeutic implication in diabetes mellitus
IF: 4.076Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2021)PMID：33663304Reactivity：MouseSample Type：Serum
实验操作视频
