Mouse LEP(Leptin) ELISA Kit
中文名称：小鼠瘦素(LEP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠LEP抗体包被于酶标板上，实验时样品（或标准品）中的小鼠LEP会与包被抗体结合。后依次加入生物素化的抗小鼠LEP抗体和辣根过氧化物酶标记的亲和素，抗小鼠LEP抗体与结合在包被抗体上的小鼠LEP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LEP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LEP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠LEP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Hypothermic Effect of Acute Citral Treatment during LPS-induced Systemic Inflammation in Obese Mice: Reduction of Serum TNF-α and Leptin Levels
IF: 4.082 -
Berberine attenuated olanzapine-induced metabolic alterations in mice: Targeting transient receptor potential vanilloid type 1 and 3 channels
IF: 3.647 -
Role of TRPV1/TRPV3 channels in olanzapine-induced metabolic alteration: Possible involvement in hypothalamic energy-sensing, appetite regulation, inflammation and mesolimbic pathway
IF: 3.347Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2020)PMID：32652086Reactivity：MouseSample Type：Serum -
Pancreastatin inhibitor PSTi8 protects the obesity associated skeletal muscle insulin resistance in diet induced streptozotocin-treated diabetic mice
IF: 3.263 -
Astragalin, (3-O-glucoside of kaempferol), isolated from Moringa oleifera leaves modulates leptin, adiponectin secretion and inhibits adipogenesis in 3T3-L1 adipocytes
IF: 2.575 -
Neonicotinoid insecticides exposure cause amino acid metabolism disorders, lipid accumulation and oxidative stress in ICR mice
IF: 5.108 -
Modeling of antipsychotic-induced metabolic alterations in mice: An experimental approach precluding psychosis as a predisposing factor
IF: 3.585Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2019)PMID：31254565Reactivity：MouseSample Type：Serum -
The protective effects of Chinese yam polysaccharide against obesity-induced insulin resistance
IF: 3.197Journal： Journal of Functional Foods (2019)Reactivity：MouseSample Type：Serum -
Virgin Grape Seed Oil Alleviates Insulin Resistance and Energy Metabolism Disorder in Mice Fed a High-Fat Diet
IF: 1.852Journal： EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY (2019)Reactivity：MouseSample Type：Serum -
Procyanidin B2 ameliorates free fatty acids-induced hepatic steatosis through regulating TFEB-mediated lysosomal pathway and redox state
IF: 6.020
实验操作视频
