Mouse LH(Luteinizing Hormone) ELISA Kit
中文名称：小鼠促黄体生成激素(LH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠LH抗体包被于酶标板上，实验时样品（或标准品）中的小鼠LH会与包被抗体结合。后依次加入生物素化的抗小鼠LH抗体和辣根过氧化物酶标记的亲和素，抗小鼠LH抗体与结合在包被抗体上的小鼠LH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠LH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Exposure to 6PPD-Q induces dysfunctions of ovarian granulosa cells: its potential role in PCOS
IF: 12.200Journal： JOURNAL OF HAZARDOUS MATERIALS (2024)Reactivity：MouseSample Type：serum -
Co-exposure of microcystin and nitrite enhanced spermatogenic disorders: The role of mtROS-mediated pyroptosis and apoptosis
IF: 10.300Journal： ENVIRONMENT INTERNATIONAL (2024)Reactivity：MouseSample Type：serum -
Collagen (peptide) extracted from sturgeon swim bladder: Physicochemical characterization and protective effects on cyclophosphamide-induced premature ovarian failure in mice
IF: 8.500Journal： FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：serum -
Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice
IF: 8.400Journal： Cell Communication and Signaling (2024)PMID：38491517Reactivity：MouseSample Type：plasma,prostate tissue -
High-fat and high-sucrose diet impairs female reproduction by altering ovarian transcriptomic and metabolic signatures
IF: 7.400 -
Silica nanoparticles cause ovarian dysfunction and fertility decrease in mice via oxidative stress-activated autophagy and apoptosis
IF: 6.200Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)Reactivity：MouseSample Type：Serum -
Study on the effects and mechanisms of Wenzhong Bushen Formula in improving ovarian reserve decline in mice based on network pharmacology
IF: 5.400 -
Mechanistic insights into EGCG's preventive effects on obesity-induced precocious puberty through multi-omics analyses
IF: 5.100 -
Luteolin and its analog luteolin-7-methylether from Leonurus japonicus Houtt suppress aromatase-mediated estrogen biosynthesis to alleviate polycystic ovary syndrome by the inhibition of tumor progression locus 2
IF: 4.800Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
Chinese medicine compound prescription HeQi San ameliorates chronic inflammatory states and modulates gut flora in dehydroepiandrosterone-induced polycystic ovary syndrome mouse model
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：Serum
实验操作视频
