Mouse LH(Luteinizing Hormone) ELISA Kit
中文名称：小鼠促黄体生成激素(LH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠LH抗体包被于酶标板上，实验时样品（或标准品）中的小鼠LH会与包被抗体结合。后依次加入生物素化的抗小鼠LH抗体和辣根过氧化物酶标记的亲和素，抗小鼠LH抗体与结合在包被抗体上的小鼠LH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠LH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Knockdown of type 2 orexin receptor in adult mouse testis potentiates testosterone production and germ cell proliferation
IF: 3.8Journal： MOLECULAR AND CELLULAR ENDOCRINOLOGY (2024)Reactivity：MouseSample Type：serum -
Human umbilical cord mesenchymal stem cell-derived extracellular vesicles harboring IGF-1 improve ovarian function of mice with premature ovarian insufficiency through the Nrf2/HO-1 pathway
IF: 3.8Journal： Journal of Ovarian Research (2024)Reactivity：MouseSample Type：serum -
Genetic loss of Uchl1 leads to female infertility by affecting oocyte quality and follicular development
IF: 3.8Journal： MOLECULAR AND CELLULAR ENDOCRINOLOGY (2024)Reactivity：MouseSample Type：serum -
The effect of gonadotropin-inhibitory hormone on steroidogenesis and spermatogenesis by acting through the hypothalamic–pituitary–testis axis in mice
IF: 3.7 -
Apelin receptor modulation mitigates letrozole-induced polycystic ovarian pathogenesis in mice
IF: 3.7 -
Maternal androgen exposure induces intergenerational effects via paternal inheritance
IF: 3.4 -
Effects of chronic CuNPs treatment followed by termination for two spermatogenic cycles in the testicular functions of mice
IF: 3.3Journal： REPRODUCTIVE TOXICOLOGY (2024)Reactivity：MouseSample Type：serum -
Mixed exposure to PFOA and PFOS induces oocyte apoptosis and subfertility in mice by activating the Hippo signaling pathway
IF: 3.3Journal： REPRODUCTIVE TOXICOLOGY (2024)Reactivity：MouseSample Type：Serum -
Sub-acute bisphenol A exposure induces proteomic alterations and impairs male reproductive health in mice
IF: 3.2Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2024)Reactivity：MouseSample Type：serum -
The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period
IF: 2.5Journal： Journal of Integrative Neuroscience (2024)Reactivity：MouseSample Type：Serum
实验操作视频
