Mouse LH(Luteinizing Hormone) ELISA Kit
中文名称：小鼠促黄体生成激素(LH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠LH抗体包被于酶标板上，实验时样品（或标准品）中的小鼠LH会与包被抗体结合。后依次加入生物素化的抗小鼠LH抗体和辣根过氧化物酶标记的亲和素，抗小鼠LH抗体与结合在包被抗体上的小鼠LH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠LH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Catechins from oolong tea improve uterine defects by inhibiting STAT3 signaling in polycystic ovary syndrome mice
IF: 2.960 -
Adoptive transfers of CD4+CD25+ Tregs partially alleviate mouse premature ovarian insufficiency
IF: 2.823Journal： MOLECULAR REPRODUCTION AND DEVELOPMENT (2020)PMID：32741069Reactivity：MouseSample Type：Serum -
High-fat diet leads to elevated lipid accumulation and endoplasmic reticulum stress in oocytes, causing poor embryo development
IF: 1.721Journal： REPRODUCTION FERTILITY AND DEVELOPMENT (2020)DOI：10.1071/RD20112PMID：32998795Reactivity：MouseSample Type：blood -
The intake of an extract from seeds of Tamarindus indica L. modulates the endocrine function of adult male mice under a high fat diet
-
Role of PCSK9 in lipid metabolic disorders and ovarian dysfunction in polycystic ovary syndrome
IF: 6.513Journal： METABOLISM-CLINICAL AND EXPERIMENTAL (2019)PMID：30768966Reactivity：MouseSample Type：serum -
miR-505-3p is a repressor of puberty onset in female mice
IF: 4.381 -
miR-29 family regulates the puberty onset mediated by a novel Gnrh1 transcription factor TBX21
IF: 4.381 -
Gestational exposure to an epidemiologically defined mixture of phthalates leads to gonadal dysfunction in mouse offspring of both sexes
IF: 4.011 -
Fluted pumpkin seeds protect against busulfan-induced oxidative stress and testicular injuries in adult mice
IF: 1.946 -
In utero exposure to acetaminophen and ibuprofen leads to intergenerational accelerated reproductive aging in female mice
实验操作视频
