Mouse MAU(Microalbuminuria) ELISA Kit
中文名称：小鼠尿微量白蛋白(MAU)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠MAU抗体包被于酶标板上，实验时样品（或标准品）中的小鼠MAU会与包被抗体结合。后依次加入生物素化的抗小鼠MAU抗体和辣根过氧化物酶标记的亲和素，抗小鼠MAU抗体与结合在包被抗体上的小鼠MAU结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MAU浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MAU的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠MAU，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Senescent renal tubular epithelial cells activate fibroblasts by secreting Shh to promote the progression of diabetic kidney disease
IF: 3.900 -
Sinensetin ameliorates high glucose-induced diabetic nephropathy via enhancing autophagy in vitro and in vivo
IF: 3.600Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2023)PMID：37393522Reactivity：MouseSample Type：urine -
BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease
IF: 9.685 -
Abelmoschus Manihot ameliorates the levels of circulating metabolites in diabetic nephropathy by modulating gut microbiota in non-obese diabetes mice
IF: 6.575 -
Bisdemethoxycurcumin Attenuated Renal Injury via Activation of Keap1/Nrf2 Pathway in High-Fat Diet-Fed Mice
IF: 6.208Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)PMID：35806399Reactivity：MouseSample Type：urinary -
Prediction of cellular targets in diabetic kidney diseases with single-cell transcriptomic analysis of db/db mouse kidneys
IF: 5.908Journal： Journal of Cell Communication and Signaling (2022)PMID：35809207Reactivity：MouseSample Type：urine -
Interaction between Plasma Metabolomics and Intestinal Microbiome in db/db Mouse, an Animal Model for Study of Type 2 Diabetes and Diabetic Kidney Disease
IF: 5.581 -
Network Pharmacology Analysis of ZiShenWan for Diabetic Nephropathy and Experimental Verification of Its Anti-Inflammatory Mechanism
IF: 4.319Journal： Drug Design Development and Therapy (2022)PMID：33883881Reactivity：HumanSample Type：Whole Blood,Urine,Tissue homogenate -
Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells
IF: 4.545 -
Local renal complement activation mediates immune kidney injury by inducing endothelin-1 signalling and inflammation in trichloroethylene-sensitised mice
IF: 3.569
实验操作视频
