Mouse MIF(Macrophage Migration Inhibitory Factor) ELISA Kit
中文名称：小鼠巨噬细胞移动抑制因子(MIF)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠MIF抗体包被于酶标板上，实验时样品（或标准品）中的小鼠MIF会与包被抗体结合。后依次加入生物素化的抗小鼠MIF抗体和辣根过氧化物酶标记的亲和素，抗小鼠MIF抗体与结合在包被抗体上的小鼠MIF结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MIF浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MIF的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠MIF，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
The role of PAR2 in regulating MIF release in house dust mite-induced atopic dermatitis
IF: 5.700 -
MIF aggravates experimental autoimmune prostatitis through activation of the NLRP3 inflammasome via the PI3K/AKT pathway
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
Macrophage inhibitory factor alters the functionality of macrophages and their involvement in disease pathogenesis of active generalized vitiligo patients
IF: 3.800 -
Acetate abates adipose-ovarian endocrinometabolic disturbance in experimentally induced polycystic ovarian syndrome
IF: 2.100Journal： STEROIDS (2024)Reactivity：RatSample Type：adipose tissue,ovarian tissue -
MIF inhibition alleviates vitiligo progression by suppressing CD8+ T cell activation and proliferation
IF: 7.300 -
Graphdiyne oxide nanosheets exert anti-lymphoma effect by killing cancer stem cells and remodeling tumor microenvironment
IF: 18.962 -
Immunological consequences of compromised ocular immune privilege accelerate retinal degeneration in retinitis pigmentosa
IF: 4.303 -
TLR7 and TLR8 agonist resiquimod (R848) differently regulates MIF expression in cells and organs
IF: 3.488
实验操作视频
