Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit
中文名称：小鼠基质金属蛋白酶9(MMP-9)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠MMP-9抗体包被于酶标板上，实验时样品（或标准品）中的小鼠MMP-9会与包被抗体结合。后依次加入生物素化的抗小鼠MMP-9抗体和辣根过氧化物酶标记的亲和素，抗小鼠MMP-9抗体与结合在包被抗体上的小鼠MMP-9结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MMP-9浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MMP-9的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠MMP-9，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Q1:ELISA可以测细胞上清液MMP-2和MMP-9吗？我看其他人写的论文，除了测这两个指标表达量，还要测活性，ELISA是不是可以测活性？跟明胶酶谱法有什么区别？
这两款试剂盒可以用于您的细胞上清的检测的。不过ELISA检测细胞上清受到培养基组分、细胞生长情况、以及外部药物刺激条件等因素的影响，建议您正式实验前先开展预实验。
明胶酶谱法是利用SDS与样品中的MMPs的可逆性结合，将样本中的MMP-2和MMP-9分离，再通过二价金属离子缓冲体系恢复两者的活性，该试验方法是专门针对MMP-2和MMP-9的活性检测的。而ELISA是通过抗原抗体的结合反应去检测待测物的含量（即浓度），即通过MMP-2和MMP-9的特异性抗体去捕获样本的MMP-2和MMP-9。两者的实验原理不同，ELISA无法检测MMP-2和MMP-9的活性。
实验操作视频
