Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit
中文名称：小鼠基质金属蛋白酶9(MMP-9)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠MMP-9抗体包被于酶标板上，实验时样品（或标准品）中的小鼠MMP-9会与包被抗体结合。后依次加入生物素化的抗小鼠MMP-9抗体和辣根过氧化物酶标记的亲和素，抗小鼠MMP-9抗体与结合在包被抗体上的小鼠MMP-9结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MMP-9浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MMP-9的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠MMP-9，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The disruption of blood-brain barrier induced by long-term arsenic exposure is associated with the increase of MMP-9 and MMP-2: The characteristics are similar to those caused by senescence
IF: 5.100Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2023)PMID：37802410Reactivity：MouseSample Type：serum,cerebral cortex -
Development of Osthole-Loaded Microemulsions as a Prospective Ocular Delivery System for the Treatment of Corneal Neovascularization: In Vitro and In Vivo Assessments
IF: 4.600Journal： Pharmaceuticals (2023)PMID：37895813Reactivity：MouseSample Type：corneal neovascularization (CNV) -
Anti?metastatic activity of aromatic aminomethylidenebisphosphonates in a mouse model of 4T1 cell?derived breast cancer
IF: 4.200 -
Anti-skin aging effect of sea buckthorn proanthocyanidins in D-galactose-induced aging mice
IF: 3.900Journal： Food Science & Nutrition (2023)Reactivity：MouseSample Type：skin tissue -
IRE1α/XBP-1 promotes β-catenin signaling activation of airway epithelium in lipopolysaccharide-induced acute lung injury
IF: 3.200Journal： PULMONARY PHARMACOLOGY & THERAPEUTICS (2023)PMID：37935327Reactivity：MouseSample Type：bronchoalveolar lavage fluid -
Twelve novel sesquiterpenes with anti-inflammatory and cholesterol-lowering activities from burdock leaves
IF: 5.307 -
Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway
IF: 2.650Journal： Evidence-based Complementary and Alternative Medicine (2022)PMID：35178108Reactivity：MouseSample Type：serum -
G Protein–Coupled Estrogen Receptor 30 Reduces Transverse Aortic Constriction–Induced Myocardial Fibrosis in Aged Female Mice by Inhibiting the ERK1/2 -MMP-9 Signaling Pathway
IF: 5.811 -
Expression of Matrix Metalloproteinases and Tissue Inhibitor of Matrix Metalloproteinases during Apical Periodontitis Development
IF: 4.171Journal： JOURNAL OF ENDODONTICS (2021)PMID：33895237Reactivity：MouseSample Type：Tissue homogenate(periapical) -
Arbutin protects brain against middle cerebral artery occlusion-reperfusion (MCAo/R) injury
IF: 3.575Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2021)PMID：34507065Reactivity：MouseSample Type：brain
实验操作视频
