Mouse MT(Melatonin) ELISA Kit
中文名称：小鼠褪黑素(MT)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠MT抗原包被于酶标板上，实验时样品或标准品中的小鼠MT与包被的小鼠MT竞争生物素标记的抗小鼠MT单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，小鼠MT浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中小鼠MT的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠MT，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Melatonin-coated nanofiber cell sheets promote periodontal regeneration through ROS scavenging and preservation of stemness
IF: 13.300 -
Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice
IF: 8.400Journal： Cell Communication and Signaling (2024)PMID：38491517Reactivity：MouseSample Type：plasma,prostate tissue -
OPA1 Mediated Fatty Acid β-Oxidation in Hepatocyte: The Novel Insight for Melatonin Attenuated Apoptosis in Concanavalin A Induced Acute Liver Injury
IF: 8.300 -
Cellular iron depletion enhances behavioral rhythm by limiting brain Per1 expression in mice
IF: 5.500 -
Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload
IF: 10.300 -
Shift and longtime light induces endometrioid adenocarcinoma via activation of PKC-α/Akt pathway in female golden hamster: Involvement of altered Aanat and Bmal1 rhythm
IF: 10.300 -
A novel combination of sitagliptin and melatonin ameliorates T2D manifestations: studies on experimental diabetic models
IF: 5.400Journal： JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION (2023)PMID：36692817Reactivity：MouseSample Type：Plasma -
Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway
IF: 7.666 -
Melatonin regulates the immune response and improves Sj?gren's syndrome-like symptoms in NOD/Ltj Mice.
IF: 6.100 -
Alterations in Gut Microbiota and Upregulations of VPAC2 and Intestinal Tight Junctions Correlate with Anti-Inflammatory Effects of Electroacupuncture in Colitis Mice with Sleep Fragmentation
IF: 5.168
实验操作视频
