Mouse NE/ELA2(Neutrophil Elastase/Elastase-2) ELISA Kit
中文名称：小鼠中性粒细胞弹性蛋白酶(NE/ELA2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠NE/ELA2抗体包被于酶标板上，实验时样品（或标准品）中的小鼠NE/ELA2会与包被抗体结合。后依次加入生物素化的抗小鼠NE/ELA2抗体和辣根过氧化物酶标记的亲和素，抗小鼠NE/ELA2抗体与结合在包被抗体上的小鼠NE/ELA2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，NE/ELA2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中NE/ELA2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠NE/ELA2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Inhibition of sympathetic tone via hypothalamic descending pathway propagates glucocorticoid-induced endothelial impairment and osteonecrosis of the femoral head
IF: 14.300Journal： Bone Research (2024)Reactivity：MouseSample Type：serum,femoral head bone marrow tissue -
Qingke Pingchuan granules alleviate airway inflammation in COPD exacerbation by inhibiting neutrophil extracellular traps in mice
IF: 6.700Journal： PHYTOMEDICINE (2024)Reactivity：Human,MouseSample Type：bronchoalveolar lavage fluid (BALF) -
Discovery of a potent, Highly selective, and In vivo anti-inflammatory Efficacious, P2Y6R antagonist with a novel quinoline-pyrazole scaffold
IF: 6.000Journal： EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF) -
LincR-PPP2R5C deficiency enhancing the fungicidal activity of neutrophils in pulmonary cryptococcosis is linked to the upregulation of IL-4
IF: 5.100Journal： mBio (2024)Reactivity：MouseSample Type：bronchoalveolar lavage fluid (BALF),lung tissue -
Didang decoction attenuates cancer-associated thrombosis by inhibiting PAD4-dependent NET formation in lung cancer
IF: 2.200 -
Nanoparticle-inhibited neutrophil elastase prevents neutrophil extracellular trap and alleviates rheumatoid arthritis in C57BL/6 mice
IF: 17.400Journal： Nano Today (2023)Reactivity：MouseSample Type：joint，serum -
Relieving thrombo-inflammation with acid-triggered polymersomes toward ischemic stroke therapy
IF: 17.400Journal： Nano Today (2023)Reactivity：MouseSample Type：brain tissue -
Neutrophil extracellular traps promote bronchopulmonary dysplasia-like injury in neonatal mice via the WNT/β-catenin pathway
IF: 5.700Journal： Frontiers in Cellular and Infection Microbiology (2023)PMID：37180448Reactivity：MouseSample Type：lung tissue -
Serotonin/5-HT7 receptor provides an adaptive signal to enhance pigmentation response to environmental stressors through cAMP-PKA-MAPK, Rab27a/RhoA, and PI3K/AKT signaling pathways
IF: 4.800 -
Hyperactive neutrophils infiltrate vital organs of tumor bearing host and contribute to gradual systemic deterioration via upregulated NE, MPO and MMP-9 activity
IF: 3.685Journal： IMMUNOLOGY LETTERS (2021)PMID：34890699Reactivity：MouseSample Type：serum, ascites, liver, lungs, spleen, peritoneum
实验操作视频
